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Anti-EB3 Antibody (1C439)
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产品编号 TMAC-01214
别名 RP3, microtubule-associated protein, RP/EB family, member 3, EBF3-S, EBF3, EB3
Anti-EB3 Antibody (1C439) 是一种 Rabbit 抗体，靶向 EB3。Anti-EB3 Antibody (1C439) 可用于 WB,ICC,IF,IHC,FCM。
Anti-EB3 Antibody (1C439)
Anti-EB3 Antibody (1C439)
还可以产品编号 TMAC-01214 别名 RP3, microtubule-associated protein, RP/EB family, member 3, EBF3-S, EBF3, EB3
Anti-EB3 Antibody (1C439) 是一种 Rabbit 抗体，靶向 EB3。Anti-EB3 Antibody (1C439) 可用于 WB,ICC,IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,495 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-EB3 Antibody (1C439) is a Rabbit antibody targeting EB3. Anti-EB3 Antibody (1C439) can be used in WB,ICC,IF,IHC,FCM. |
| 别名 | RP3, microtubule-associated protein, RP/EB family, member 3, EBF3-S, EBF3, EB3 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of EB3 on different lysates using anti-EB3 antibody at 1/500 dilution. Positive control: Lane 1: Mouse brain tissue, Lane 2: K562. 2. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-EB3 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-EB3 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-EB3 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-EB3 antibody. Counter stained with hematoxylin. 6. ICC staining EB3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining EB3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining EB3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of SH-SY-5Y cells with EB3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | EB1 (MAPRE2, microtubule-associated protein, RP/EB family, member 2, EB2, RP1) may influence tumorigenesis of colorectal cancers and proliferative control of normal cells. EB1 may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR. Colorectal cancer is caused by the pathologic transformation of normal colonic epithelium to an adenomatous polyp, which can become an invasive cancer. APC (adenomatous polyposis coli) is a tumor suppressor gene, the mutation of which is one of the earliest events in colorectal carcinogenesis. A majority of the mutations result in the loss of the carboxy terminus of APC. EB1 has been shown to bind to the carboxy terminal region of APC, which implicates EB1 in APC suppression of colonic cancer. EB1 overexpression may play a role in the development of human esophageal squamous cell carcinoma (ESCC) by affecting APC function and activating the beta-catenin/TCF pathway. EB3 is related to EB1 and likewise associates with the microtubule cytoskeleton. EB3 is expressed predominantly in the central nervous system and preferentially associates with APCL. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 32 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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