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Anti-DYKDDDDK-Tag Polyclonal Antibody
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产品编号 TMAC-01194
Anti-DYKDDDDK-Tag Polyclonal Antibody 是一种 Rabbit 抗体，靶向 DYKDDDDK-Tag。Anti-DYKDDDDK-Tag Polyclonal Antibody 可用于 WB。
Anti-DYKDDDDK-Tag Polyclonal Antibody
Anti-DYKDDDDK-Tag Polyclonal Antibody
还可以产品编号 TMAC-01194
Anti-DYKDDDDK-Tag Polyclonal Antibody 是一种 Rabbit 抗体，靶向 DYKDDDDK-Tag。Anti-DYKDDDDK-Tag Polyclonal Antibody 可用于 WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 795 | 5日内发货 | |
| 100 μL | ¥ 1,190 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-DYKDDDDK-Tag Polyclonal Antibody is a Rabbit antibody targeting DYKDDDDK-Tag. Anti-DYKDDDDK-Tag Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| 验证活性 | 1. Western Blot analysis using FLAG-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing FLAG tag (1) and untransfected (2). Secondary antibody was diluted at 1:20000. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-3000 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Polyclonal Antibody |
| 纯化方式 | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| 性状 | Liquid |
| 缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| 研究背景 | The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. |
| 偶联 | Unconjugated |
| 免疫原 | KLH conjugated synthetic peptide: DDDDK |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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