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Anti-DUSP6 Antibody (2Q808) 是一种 Rabbit 抗体,靶向 DUSP6。Anti-DUSP6 Antibody (2Q808) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-DUSP6 Antibody (2Q808) 是一种 Rabbit 抗体,靶向 DUSP6。Anti-DUSP6 Antibody (2Q808) 可用于 WB,ICC/IF,IHC,IP,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,495 | 5日内发货 |
产品描述 | Anti-DUSP6 Antibody (2Q808) is a Rabbit antibody targeting DUSP6. Anti-DUSP6 Antibody (2Q808) can be used in WB,ICC/IF,IHC,IP,FCM. |
别名 | Serine/threonine specific protein phosphatase, PYST1, PYST 1, MKP-3, MKP3, MKP 3, Mitogen-activated protein kinase phosphatase 3, Mitogen activated protein kinase phosphatase 3, MAP kinase phosphatase 3, HH19, DUSP6a, Dusp6, DUSP 6a, DUSP 6, DUS6_HUMAN, Dual specificity protein phosphatase PYST1, Dual specificity protein phosphatase 6, Dual specificity phosphatase 6 isoform a, Dual specificity phosphatase 6 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of DUSP6 on mouse pancreas lysates using anti-DUSP6 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-DUSP6 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-DUSP6 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DUSP6 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-DUSP6 antibody. Counter stained with hematoxylin. 6. ICC staining DUSP6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining DUSP6 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining DUSP6 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of NIH/3T3 cells with DUSP6 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Mitogen-activated protein (MAP) kinases are a large class of proteins involved in signal transduction pathways that are activated by a range of stimuli and mediate a number of physiological and pathological changes in the cell. Dual specificity phosphatases (DSPs) are a subclass of the protein tyrosine phosphatase (PTP) gene superfamily, which are selective for dephosphorylating critical phosphothreonine and phosphotyrosine residues within MAP kinases. DSP gene expression is induced by a host of growth factors and/or cellular stresses, thereby negatively regulating MAP kinase superfamily members including MAPK/ERK, SAPK/JNK and p38. The members of the dual-specificity phosphatase protein family include MKP-1/CL100 (3CH134), VHR, PAC1, MKP-2, hVH-3 (B23), hVH-5, MKP-3, MKP-X, and MKP-4. Human MKP-3 maps to chromosome 12q22-q23 and encodes a 381 amino acid protein that specifically inactivates members of the ERK family and is expressed in a variety of tissues with the highest levels in heart and pancreas. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 42 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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