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Anti-CYPOR Antibody (2K509) 是一种 Rabbit 抗体,靶向 CYPOR。Anti-CYPOR Antibody (2K509) 可用于 WB,ICC,IF,IHC,FCM。
Anti-CYPOR Antibody (2K509) 是一种 Rabbit 抗体,靶向 CYPOR。Anti-CYPOR Antibody (2K509) 可用于 WB,ICC,IF,IHC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,485 | 5日内发货 | |
100 μL | ¥ 2,480 | 5日内发货 |
产品描述 | Anti-CYPOR Antibody (2K509) is a Rabbit antibody targeting CYPOR. Anti-CYPOR Antibody (2K509) can be used in WB,ICC,IF,IHC,FCM. |
别名 | POR, P450R, NADPH--cytochrome P450 reductase, CYPOR, CPR |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of Cytochrome P450 Reductase on SK-Br-3 (1) , A549 (2) and mouse lung (3) lysate using anti-Cytochrome P450 Reductase antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytochrome P450 Reductase antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cytochrome P450 Reductase antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cytochrome P450 Reductase antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cytochrome P450 Reductase antibody. Counter stained with hematoxylin. 6. ICC staining Cytochrome P450 Reductase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Cytochrome P450 Reductase in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining Cytochrome P450 Reductase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of HepG2 cells with Cytochrome P450 Reductase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. Several P450 enzymes have been classified by sequence similarities as members of the CYP1A and CYP2A subfamilies. CYPOR, also known as cytochrome P450 reductase and NADPH cytochrome P450 reductase, is a microsomal enzyme responsible for the transfer of electrons from NADPH to cytochrome P450 enzymes during the P450 catalytic cycle. CYPOR is localized to the endoplasmic reticulum, where it is also able to transfer electrons to heme oxygenase and cytochrome b5. CYPOR is structurally related to two separate flavoprotein families, ferredoxin nucleotide reductase (FNR) and flavodoxin. Electron transfer of CYPOR requires the binding of two flavin cofactors, FAD and FMN, to the FNR and flavodoxin domains, respectively. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 77 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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