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Anti-Cyclin H Antibody (8F15) 是一种 Rabbit 抗体,靶向 Cyclin H。Anti-Cyclin H Antibody (8F15) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-Cyclin H Antibody (8F15) 是一种 Rabbit 抗体,靶向 Cyclin H。Anti-Cyclin H Antibody (8F15) 可用于 WB,ICC/IF,IHC,IP,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,485 | 5日内发货 |
产品描述 | Anti-Cyclin H Antibody (8F15) is a Rabbit antibody targeting Cyclin H. Anti-Cyclin H Antibody (8F15) can be used in WB,ICC/IF,IHC,IP,FCM. |
别名 | p37, p36, p34, MO15-associated protein, MO15 associated protein, Cyclin-H, CyclinH, Cyclin H, cyclin dependent kinase activating kinase complex subunit, Cyclin dependent kinase activating kinase, CDK activating kinase complex subunit, CDK activating kinase, CCNH_HUMAN, ccnh, CAK complex subunit, CAK, AW538719, AV102684, AI661354, 6330408H09Rik |
Ig Type | IgG |
克隆号 | 8F15 |
交叉反应 | Human,Mouse |
验证活性 | 1. Western blot analysis of Cyclin H on K562 cells lysates using anti-Cyclin H antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cyclin H antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cyclin H antibody. Counter stained with hematoxylin. 4. ICC staining Cyclin H in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Cyclin H in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Cyclin H in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Hela cells with Cyclin H antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Progression through the cell cycle requires activation of a series of enzymes designated cyclin dependent kinases (Cdks). The monomeric catalytic subunit, Cdk2, a critical enzyme for initiation of cell cycle progression, is completely inactive. Partial activation is achieved by the binding of regulatory cyclins such as cyclin D1, while full activation requires, in addition, phosphorylation at Thr-160. The enzyme responsible for phosphorylation of Thr-160 in Cdk2 and also Thr-161 in Cdc2 p34, designated Cdk-activating kinase (CAK), has been partially purified and shown to be comprised of a catalytic subunit and a regulatory subunit. The catalytic subunit, designated Cdk7, has been identified as the mammalian homolog of MO15, a protein kinase demonstrated earlier in starfish and Xenopus. The regulatory subunit is a novel cyclin (cyclin H) and is required for activation of Cdk7. Like other Cdks, Cdk7 contains a conserved threonine required for full activity; mutation of this residue severely reduces CAK activity. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 38 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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