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Anti-Cullin 1 Antibody (2P159)
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产品编号 TMAC-01018
别名 MGC149835, MGC149834, Cullin-1, Cullin1, CUL1_HUMAN, CUL-1, CUL1, CUL 1
Anti-Cullin 1 Antibody (2P159) 是一种 Rabbit 抗体，靶向 Cullin 1。Anti-Cullin 1 Antibody (2P159) 可用于 WB,IHC,IP,FCM,ICC/IF。
Anti-Cullin 1 Antibody (2P159)
Anti-Cullin 1 Antibody (2P159)
还可以产品编号 TMAC-01018 别名 MGC149835, MGC149834, Cullin-1, Cullin1, CUL1_HUMAN, CUL-1, CUL1, CUL 1
Anti-Cullin 1 Antibody (2P159) 是一种 Rabbit 抗体，靶向 Cullin 1。Anti-Cullin 1 Antibody (2P159) 可用于 WB,IHC,IP,FCM,ICC/IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,495 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Cullin 1 Antibody (2P159) is a Rabbit antibody targeting Cullin 1. Anti-Cullin 1 Antibody (2P159) can be used in WB,IHC,IP,FCM,ICC/IF. |
| 别名 | MGC149835, MGC149834, Cullin-1, Cullin1, CUL1_HUMAN, CUL-1, CUL1, CUL 1 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of Cullin 1 on MCF-7 cell using anti-Cullin 1 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cullin 1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Cullin 1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse oviduct tissue using anti-Cullin 1 antibody. Counter stained with hematoxylin. 5. Flow cytometric analysis of Hela cells with Cullin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC/IF: 1:10-50; IP: 1:10-50; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, also designated vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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