Anti-CTH Polyclonal Antibody is a Rabbit antibody targeting CTH. Anti-CTH Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Dog,Cow,Rabbit)

1. Sample: K562 Cell (Human) Lysate at 40 μg
Primary: Anti-CTH (TMAB-00490) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
2. Sample: Esophagus (Mouse) Lysate at 40 μg
Primary: Anti-CTH (TMAB-00490) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
3. Sample: Liver (Mouse) Lysate at 40 μg
Primary: Anti-CTH (TMAB-00490) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 48 kDa
4. Blank control: U937. Primary Antibody (green line): Rabbit Anti-CTH antibody (TMAB-00490)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 2ug/Test

Polyclonal

KLH conjugated synthetic peptide: human CTH/CSE/Cystathionase

Human

Amino acid metabolism,Amino Acids