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顶部生物活性储存和溶解信息计算器

Anti-CRISPR-Cas9 Antibody (4K140)

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Anti-CRISPR-Cas9 Antibody (4K140)
产品编号 TMAC-00979
别名 SpyCas9

Anti-CRISPR-Cas9 Antibody (4K140) 是一种 Rabbit 抗体，靶向 CRISPR-Cas9。Anti-CRISPR-Cas9 Antibody (4K140) 可用于 WB,IHC,ICC/IF,FCM。

Anti-CRISPR-Cas9 Antibody (4K140)

Anti-CRISPR-Cas9 Antibody (4K140)

Rating icon 还可以
Anti-CRISPR-Cas9 Antibody (4K140)
产品编号 TMAC-00979 别名 SpyCas9

Anti-CRISPR-Cas9 Antibody (4K140) 是一种 Rabbit 抗体，靶向 CRISPR-Cas9。Anti-CRISPR-Cas9 Antibody (4K140) 可用于 WB,IHC,ICC/IF,FCM。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,490
5日内发货
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产品介绍

生物活性
产品描述
Anti-CRISPR-Cas9 Antibody (4K140) is a Rabbit antibody targeting CRISPR-Cas9. Anti-CRISPR-Cas9 Antibody (4K140) can be used in WB,IHC,ICC/IF,FCM.
别名SpyCas9
Ig Type
IgG
交叉反应
Recombinant Cas9 Streptococcus pyogenes
验证活性
1. Western blot analysis of CRISPR-Cas9 SP expression in (1) 293T cell lysate transfected with CRISPR-Cas9; (2) 293T cell lysate; (3) 3T3 cell lysate; (4) PC12 cell lysate.
2. Immunofluorescent analysis of 293T cells transfected with CRISPR-SpCas9, using CRISPR-Cas9 SP Antibody .
应用
WB
IHC
ICC/IF
FCM
推荐剂量
WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式Affinity-chromatography
性状Liquid
缓冲液Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant fragment derived from Streptococcus pyogenes
Uniprot ID
Q99ZW2
化学信息
分子量Theoretical: 158 kDa.
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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