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Anti-Clusterin Antibody (9C938)
还可以产品编号 TMAY-01834
Anti-Clusterin Antibody (9C938) 是一种 Mouse 抗体，靶向 Clusterin。Anti-Clusterin Antibody (9C938) 可用于 WB,ELISA,FCM,ICC/IF。
Anti-Clusterin Antibody (9C938)
Anti-Clusterin Antibody (9C938)
还可以产品编号 TMAY-01834
Anti-Clusterin Antibody (9C938) 是一种 Mouse 抗体，靶向 Clusterin。Anti-Clusterin Antibody (9C938) 可用于 WB,ELISA,FCM,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Clusterin Antibody (9C938) is a Mouse antibody targeting Clusterin. Anti-Clusterin Antibody (9C938) can be used in WB,ELISA,FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 9C938 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human CLU in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human CLU monoclonal antibody (1:300) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human CLU expression on A549 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human CLU, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 3. Anti-CLU mouse monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:52 kDa. -Observed band size:57 kDa |
| 应用 | WB,ELISA,FCM,ICC/IF |
| 推荐剂量 | WB: 1:500-1:1000; ELISA: 1:5000-1:10000; ICC-IF: 1:100-1:500; FCM: 1:100-1:500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Clusterin / Apolipoprotein J / Apo-J (rh Clusterin / Apolipoprotein J / Apo-J; TMPY-01691; NP_001822.2; Met 1-Glu 501). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Clusterin, also known as complement-associated protein SP-40, Complement cytolysis inhibitor, Apolipoprotein J, Testosterone-repressed prostate message 2, Aging-associated gene 4 protein, CLU and APOJ, is a secreted protein which belongs to the clusterin family. Clusterin/Apolipoprotein J/Apo-J is an enigmatic glycoprotein with a nearly ubiquitous tissue distribution and an apparent involvement in biological processes ranging from mammary gland involution to neurodegeneration in Alzheimer's disease. Its major form, a heterodimer, is secreted and present in physiological fluids, but truncated forms targeted to the nucleus have also been identified. Clusterin/Apolipoprotein J/Apo-J is a widely distributed glycoprotein with a wide range of biologic properties. A prominent and defining feature of clusterin is its marked induction in such disease states as glomerulonephritis, cystic renal disease, renal tubular injury, neurodegenerative conditions, atherosclerosis, and myocardial infarction. Upregulation of clusterin mRNA and protein levels detected in diverse disease states and in in vitro systems have led to suggestions that it functions in membrane lipid recycling, in apoptotic cell death, and as a stress-induced secreted chaperone protein, amongst others. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | FITC/PE |
| 可提供抗体种类 | 3 |
| 免疫原 | Recombinant Human Clusterin / Apolipoprotein J / Apo-J Protein (TMPY-01691) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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