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Anti-CHEK2 Antibody (3L509)

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Anti-CHEK2 Antibody (3L509)
产品编号 TMAC-00848
别名 RAD53, PP1425, LFS2, HuCds1, hCds1, CHK2, checkpoint kinase 2, CDS1

Anti-CHEK2 Antibody (3L509) 是一种 Rabbit 抗体,靶向 CHEK2。Anti-CHEK2 Antibody (3L509) 可用于 WB,ICC/IF,IHC,IP。

Anti-CHEK2 Antibody (3L509)

Anti-CHEK2 Antibody (3L509)

Rating icon 还可以
Anti-CHEK2 Antibody (3L509)
产品编号 TMAC-00848 别名 RAD53, PP1425, LFS2, HuCds1, hCds1, CHK2, checkpoint kinase 2, CDS1

Anti-CHEK2 Antibody (3L509) 是一种 Rabbit 抗体,靶向 CHEK2。Anti-CHEK2 Antibody (3L509) 可用于 WB,ICC/IF,IHC,IP。

规格价格库存数量
50 μL
¥ 1,495
5日内发货
100 μL
¥ 2,485
5日内发货
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产品介绍

生物活性
产品描述
Anti-CHEK2 Antibody (3L509) is a Rabbit antibody targeting CHEK2. Anti-CHEK2 Antibody (3L509) can be used in WB,ICC/IF,IHC,IP.
别名RAD53, PP1425, LFS2, HuCds1, hCds1, CHK2, checkpoint kinase 2, CDS1
Ig Type
IgG
交叉反应
Human
验证活性
1. Western blot analysis of Chk2 on Hela cells lysates using anti-Chk2 antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Chk2 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Chk2 antibody. Counter stained with hematoxylin.
4. ICC staining Chk2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining Chk2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining Chk2 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
应用
推荐剂量
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
化学信息
分子量Theoretical: 62 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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