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Anti-CDK6 Antibody (5T828)
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产品编号 TMAC-00797
别名 Serine/threonine-protein kinase PLSTIRE, EC 2.7.11.22, Cyclin-dependent kinase 6, Cell division protein kinase 6, CDKN6, CDK 6
Anti-CDK6 Antibody (5T828) 是一种 Rabbit 抗体，靶向 CDK6。Anti-CDK6 Antibody (5T828) 可用于 WB,ICC/IF,IHC,FCM。
Anti-CDK6 Antibody (5T828)
Anti-CDK6 Antibody (5T828)
还可以产品编号 TMAC-00797 别名 Serine/threonine-protein kinase PLSTIRE, EC 2.7.11.22, Cyclin-dependent kinase 6, Cell division protein kinase 6, CDKN6, CDK 6
Anti-CDK6 Antibody (5T828) 是一种 Rabbit 抗体，靶向 CDK6。Anti-CDK6 Antibody (5T828) 可用于 WB,ICC/IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
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产品介绍
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偶联与修饰
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| 产品描述 | Anti-CDK6 Antibody (5T828) is a Rabbit antibody targeting CDK6. Anti-CDK6 Antibody (5T828) can be used in WB,ICC/IF,IHC,FCM. |
| 别名 | Serine/threonine-protein kinase PLSTIRE, EC 2.7.11.22, Cyclin-dependent kinase 6, Cell division protein kinase 6, CDKN6, CDK 6 |
| Ig Type | IgG |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of Cdk6 on different lysates using anti-Cdk6 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat, Lane 2: K562, Lane 3: Hela. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cdk6 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cdk6 antibody. Counter stained with hematoxylin. 4. ICC staining Cdk6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Cdk6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Cdk6 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Hela cells with Cdk6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression. Another family of proteins, Cdk inhibitors, also plays a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1-PCTAIRE-3, PITALRE and PITSLRE. Cdk6 is known to associate with cyclins D1, D2 and D3 and to be involved with the G1/S transition of the cell cycle. Multiple inhibitors of Cdk6 have been identified, including p18 and p19. These inhibitors bind to both free and complexed Cdk6, and they inhibit the activity of the cyclin D-bound Cdk6. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 37 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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