Anti-CD73 Antibody (3N745) is a Mouse antibody targeting CD73. Anti-CD73 Antibody (3N745) can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Mouse IgG1

3N745

Human

1. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD73) Monoclonal Antibody, Unconjugated (TMAB-04060) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD73) Monoclonal Antibody, Unconjugated (TMAB-04060) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD73) Monoclonal Antibody, Unconjugated (TMAB-04060) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD73) Monoclonal Antibody, Unconjugated (TMAB-04060) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD73) Monoclonal Antibody, Unconjugated (TMAB-04060) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
6. Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human A375 cell lysates
Primary: Anti-CD73 (TMAB-04060) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa
7. Blank control: A375.
Primary Antibody (green line): Mouse Anti-CD73 antibody (TMAB-04060)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line) : Goat anti-Mouse IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Mouse IgG
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; IHC-P: 1:200-400; IHC-Fr: 1:400-800; IF: 1:100-500; FCM: 1μg/Test

Monoclonal

Recombinant Protein: human CD73 protein

Human

Nt5e

5'-nucleotidase

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.