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Anti-CD59 Antibody (2A586)
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产品编号 TMAC-00724
别名 MSK21, MIRL, MIN3, MIN2, MIN1, MIC11, MEM43, MAC-IP, MACIP, MACIF, HRF-20, HRF20, G344, CD59, 1F5
Anti-CD59 Antibody (2A586) 是一种 Rabbit 抗体，靶向 CD59。Anti-CD59 Antibody (2A586) 可用于 WB,ICC/IF,FCM,IP。
Anti-CD59 Antibody (2A586)
Anti-CD59 Antibody (2A586)
还可以产品编号 TMAC-00724 别名 MSK21, MIRL, MIN3, MIN2, MIN1, MIC11, MEM43, MAC-IP, MACIP, MACIF, HRF-20, HRF20, G344, CD59, 1F5
Anti-CD59 Antibody (2A586) 是一种 Rabbit 抗体，靶向 CD59。Anti-CD59 Antibody (2A586) 可用于 WB,ICC/IF,FCM,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,495 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-CD59 Antibody (2A586) is a Rabbit antibody targeting CD59. Anti-CD59 Antibody (2A586) can be used in WB,ICC/IF,FCM,IP. |
| 别名 | MSK21, MIRL, MIN3, MIN2, MIN1, MIC11, MEM43, MAC-IP, MACIP, MACIF, HRF-20, HRF20, G344, CD59, 1F5 |
| Ig Type | IgG |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of CD59 on different cells lysates using anti-CD59 antibody at 1/500 dilution. Positive control: Lane 1: Human placenta, Lane 2: HUVEC, Lane 3: K562. 2. ICC staining CD59 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining CD59 in JAR cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining CD59 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of NIH-3T3 cells with CD59 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000; ICC/IF: 1:100-500; IP: 1:10-50; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | CD59 is a GPI-anchored glycoprotein that is expressed on leukocytes, vascular endothelial cells, various epithelial cells and placenta. CD59 acts together with CD58 in mediating T cell adhesion and activation, and it may be a second ligand of CD2. CD59 functions as a regulator of the terminal pathway of complement by binding to the C8/C9 components of the assembling membrane attack complex (MAC) on host cell membranes, to stop the formation of the lytic pore. CD59 also drives both calcium release and activation of lipid-raft associated signalling molecules such as tyrosine kinases. CD59 gene has two p53-responsive domains that may be implicated in the defense of host cells from damage by the complement system in inflammation, suggesting that p53 could be used to mediate susceptibility of tumor cells to the complement lysis during chemotherapy. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 14/20 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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