Anti-CD3E Antibody (1S389) is a Mouse antibody targeting CD3E. Anti-CD3E Antibody (1S389) can be used in IF, IHC-Fr, IHC-P, WB.

IgG

1S389

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with CD3E monoclonal antibody, unconjugated (TMAB-03975) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD3E Monoclonal Antibody, Unconjugated (TMAB-03975) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD3E Monoclonal Antibody, Unconjugated (TMAB-03975) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD3E Monoclonal Antibody, Unconjugated (TMAB-03975) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD3E Monoclonal Antibody, Unconjugated (TMAB-03975) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD3E Monoclonal Antibody, Unconjugated (TMAB-03975) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD3E Monoclonal Antibody, Unconjugated (TMAB-03975) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD3E Monoclonal Antibody, Unconjugated (TMAB-03975) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD3E Monoclonal Antibody, Unconjugated (TMAB-03975) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD3E Monoclonal Antibody, Unconjugated (TMAB-03975) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD3E Monoclonal Antibody, Unconjugated (TMAB-03975) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human appendix; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD3E Monoclonal Antibody, Unconjugated (TMAB-03975) at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.

IF=1:200-800; IHC-Fr=1:200-800; IHC-P=1:200-800; WB=1:1000-2000

Monoclonal

KLH conjugated synthetic peptide: mouse CD3E

Mouse

CD3E

T-cell surface glycoprotein CD3 epsilon chain

The CD3 complex mediates signal transduction.