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Anti-CD27/TNFRSF7 Antibody (4I709)
还可以产品编号 TMAY-03108
Anti-CD27/TNFRSF7 Antibody (4I709) 是一种 Rabbit 抗体，靶向 CD27/TNFRSF7。Anti-CD27/TNFRSF7 Antibody (4I709) 可用于 FCM,ICC/IF。
Anti-CD27/TNFRSF7 Antibody (4I709)
Anti-CD27/TNFRSF7 Antibody (4I709)
还可以产品编号 TMAY-03108
Anti-CD27/TNFRSF7 Antibody (4I709) 是一种 Rabbit 抗体，靶向 CD27/TNFRSF7。Anti-CD27/TNFRSF7 Antibody (4I709) 可用于 FCM,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-CD27/TNFRSF7 Antibody (4I709) is a Rabbit antibody targeting CD27/TNFRSF7. Anti-CD27/TNFRSF7 Antibody (4I709) can be used in FCM,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 4I709 |
| 交叉反应 | Mouse |
| 验证活性 | 1. Immunofluorescence staining of Mouse CD27 in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD27 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane. 2. Expression of CD27 on mouse splenocytes. BABL/c splenocytes were stained with purified mAb R012, then a FITC-conjugated second step antibody. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system. |
| 应用 | FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD27 (rM CD27; TMPY-01432; NP_001028298.1; Met1-Arg182). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has an important role in the generation of T cell immunity and is an robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.Cancer ImmunotherapyCo-stimulatory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint Detection: IHC AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
| 偶联 | Unconjugated |
| 其他可选修饰符 | APC/PerCP/FITC/PE |
| 可提供抗体种类 | 5 |
| 免疫原 | Recombinant Mouse CD27 protein (TMPY-01432) |
| 抗原种属 | Mouse |
| 研究领域 | Cancer Drug Targets |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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