Anti-CD20 Polyclonal Antibody is a Rabbit antibody targeting CD20. Anti-CD20 Polyclonal Antibody can be used in WB,FCM,ELISA.

IgG

Mouse (predicted:Human,Rat)

1. Blank control: U937 (blue). Primary Antibody: Rabbit Anti-CD20 antibody (TMAB-00341), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (TMAB-00341, 1 μg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice.
2. Sample:
Lane 1: LympHnode (Mouse) Lysate at 40 μg
Lane 2: Bone (Mouse) Lysate at 40 μg
Primary: Anti-CD20 (TMAB-00341) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa

WB: 1:500-2000; FCM: 1μg /Test; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: human CD20

Human

Tumor-associated antigens,CD,Human Lineage Negative