Anti-CD19 Antibody (9V675) is a Mouse antibody targeting CD19. Anti-CD19 Antibody (9V675) can be used in ELISA, WB, IHC, FC.

IgG2b

9V675

Human

1. Western Blot
-Positive WB detected in: Raji whole cell lysate
-All lanes: CD19 antibody at 1:2000
-Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution
-Predicted band size: 61 kDa
-Observed band size: 95 kDa
2. IHC image of TMAH-00175 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
3. IHC image of TMAH-00175 diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
4. Overlay histogram showing Raji cells stained with TMAH-00175 (red line) at 1:50. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, IHC, FC

Monoclonal

Unconjugated

Recombinant Protein: Human CD19 Protein (20-291AA)

Human

930

Immunology