Anti-Catalase/CAT Polyclonal Antibody is a Rabbit antibody targeting Catalase/CAT. Anti-Catalase/CAT Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM,ELISA.

IgG

Human,Rat (predicted:Mouse,Horse)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Catalase Polyclonal Antibody, Unconjugated (TMAB-00295) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Blank control: HL60. Primary Antibody (green line): Rabbit Anti-Catalase antibody (TMAB-00295)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
3. Sample:
Lane 1: Rat Kidney tissue lysates
Lane 2: Rat Placenta tissue lysates
Lane 3: Rat Heart tissue lysates
Lane 4: Human HeLa cell lysates
Lane 5: Human HepG2 cell lysates
Primary: Anti-CAT at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: mouse Catalase

Mouse

Cellular metabolic process,Cardiac metabolism,Antioxidants,Heat Shock Proteins,Peroxisome