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Anti-Caspase-8 Antibody (7P665) 是一种 Mouse 抗体,靶向 Caspase-8。Anti-Caspase-8 Antibody (7P665) 可用于 WB。
Anti-Caspase-8 Antibody (7P665) 是一种 Mouse 抗体,靶向 Caspase-8。Anti-Caspase-8 Antibody (7P665) 可用于 WB。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,170 | 5日内发货 | |
100 μL | ¥ 1,965 | 5日内发货 | |
200 μL | ¥ 2,785 | 5日内发货 |
产品描述 | Anti-Caspase-8 Antibody (7P665) is a Mouse antibody targeting Caspase-8. Anti-Caspase-8 Antibody (7P665) can be used in WB. |
Ig Type | IgG |
克隆号 | 7P665 |
交叉反应 | Human,Mouse (predicted:Rat) |
验证活性 | 1. Sample: Hela (Human) Cell Lysate at 30 μg Jurkat (Human) Cell Lysate at 30 μg Molt-4 (Human) Cell Lysate at 30 μg K562 (Human) Cell Lysate at 30 μg Primary: Anti-Caspase 8 (TMAB-00289) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41/43/55 kDa Observed band size: 43 kDa 2. Sample: Stomach (Mouse) Lysate at 40 μg Spleen (Mouse) Lysate at 40 μg LympHnode (Mouse) Lysate at 40 μg Urinary bladder (Mouse) Lysate at 40 μg Primary: Anti-Caspase 8 (TMAB-00289) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41/43/55 kDa Observed band size: 55/53D 3. Sample: 293T-Uv (Human) Cell Lysate at 30 μg U251-Uv (Human) Cell Lysate at 30 μg Primary: Anti-Caspase 8 (TMAB-00289) at 1/4000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41/43/55 kDa Observed band size: 43 kDa |
应用 | |
推荐剂量 | WB: 1:2000-5000 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
亚细胞定位 | Cytoplasm. |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | Protein G purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. |
免疫原 | Recombinant Protein: human Caspase 8 Protein |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Caspases,Metabolism,Necroptosis,Caspases,Caspases,Other proteases,Apoptosis |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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