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顶部生物活性储存和溶解信息计算器

Anti-BMI-1 Antibody (8M743)

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产品编号 TMAC-00454

Anti-BMI-1 Antibody (8M743) 是一种 Mouse 抗体，靶向 BMI-1。Anti-BMI-1 Antibody (8M743) 可用于 FCM,ICC,IHC,WB。

Anti-BMI-1 Antibody (8M743)

Anti-BMI-1 Antibody (8M743)

一键复制产品信息
Rating icon 还可以
产品编号 TMAC-00454

Anti-BMI-1 Antibody (8M743) 是一种 Mouse 抗体，靶向 BMI-1。Anti-BMI-1 Antibody (8M743) 可用于 FCM,ICC,IHC,WB。

规格价格库存数量
50 μL
¥ 1,990
5日内发货
100 μL
¥ 2,990
5日内发货
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产品介绍

生物活性
产品描述
Anti-BMI-1 Antibody (8M743) is a Mouse antibody targeting BMI-1. Anti-BMI-1 Antibody (8M743) can be used in FCM,ICC,IHC,WB.
克隆号
8M743
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T, Lane 2: Jurkat, Lane 3: Hela,, Lane 4: MCF-7, Lane 5: HepG2, Lane 6: NIH/3T3, Lane 7: PC12, Lane 8: Mouse kidney, Lane 9: Human kidney, Lane 10: K562, Lane 11: Human brain.
2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMI1 antibody. Counter stained with hematoxylin.
5. ICC staining Bmi1 in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining Bmi1 in Lovo cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining Bmi1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. Flow cytometric analysis of Hela cells with BMI1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
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应用FCMICCIHCWB
推荐剂量
WB: 1:1000; IHC: 1:200; ICC: 1:200; FCM: 1:100-200
抗体种类
Monoclonal
宿主来源Mouse
构建方式Hybridoma Monoclonal Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景The Bmi-1 was identified initially as an oncogene that cooperates with c-myc in the generation of B-cell lymphoma. It contributes to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell-cycle arrest.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: human Bmi1 full sequence
抗原种属
human
Uniprot ID
P35226
化学信息
分子量Theoretical: 37 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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关键词

RNF51RNF 51RING finger protein 51Polycomb group RING finger protein 4Polycomb complex protein BMI-1PCGF4PCGF 4BMI1BMI 1

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4个月前
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