Anti-BMI-1 Antibody (8M743) is a Mouse antibody targeting BMI-1. Anti-BMI-1 Antibody (8M743) can be used in WB,ICC,IHC,FCM.

Human,Mouse,Rat

1. Western blot analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T, Lane 2: Jurkat, Lane 3: Hela,, Lane 4: MCF-7, Lane 5: HepG2, Lane 6: NIH/3T3, Lane 7: PC12, Lane 8: Mouse kidney, Lane 9: Human kidney, Lane 10: K562, Lane 11: Human brain.
2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMI1 antibody. Counter stained with hematoxylin.
5. ICC staining Bmi1 in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining Bmi1 in Lovo cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining Bmi1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. Flow cytometric analysis of Hela cells with BMI1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.

WB: 1:1000; IHC: 1:200; ICC: 1:200; FCM: 1:100-200

Monoclonal

Unconjugated

Recombinant Protein: human Bmi1 full sequence

human