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Anti-BCL2 Antibody (4Z465)
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产品编号 TMAC-00394
别名 PPP1R50, Bcl-2, B-cell CLL/lymphoma 2
Anti-BCL2 Antibody (4Z465) 是一种 Rabbit 抗体，靶向 BCL2。Anti-BCL2 Antibody (4Z465) 可用于 WB,ICC/IF,IHC,FCM。
Anti-BCL2 Antibody (4Z465)
Anti-BCL2 Antibody (4Z465)
还可以产品编号 TMAC-00394 别名 PPP1R50, Bcl-2, B-cell CLL/lymphoma 2
Anti-BCL2 Antibody (4Z465) 是一种 Rabbit 抗体，靶向 BCL2。Anti-BCL2 Antibody (4Z465) 可用于 WB,ICC/IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,485 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-BCL2 Antibody (4Z465) is a Rabbit antibody targeting BCL2. Anti-BCL2 Antibody (4Z465) can be used in WB,ICC/IF,IHC,FCM. |
| 别名 | PPP1R50, Bcl-2, B-cell CLL/lymphoma 2 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse |
| 验证活性 | 1. Western blot analysis of Bcl-2 on different lysates using anti-Bcl-2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: Jurkat, Lane 3: MCF-7. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Bcl-2 antibody. Counter stained with hematoxylin. 6. ICC staining Bcl-2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Bcl-2 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Jurkat cells with Bcl-2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Bcl-2 is one among many key regulators of apoptosis, which are essential for proper development, tissue homeostasis, and protection against foreign pathogens. Human Bcl-2 is an apoptotic, membrane-associated oncoprotein that can promote cell survival through protein-protein interactions with other Bcl-2 related family members, such as the death suppressors Bcl-xL, Mcl-1, Bcl-w, and A1 or the death agonists Bax, Bak, Bik, Bad, and Bid. The apoptotic function of Bcl-2 can also be regulated through proteolytic processing and phospho-rylation. Bcl-2 may promote cell survival by interfering with the activation of the cytochrome c/Apaf-1 pathway through stabilization of the mitochondrial membrane. Mutations in the Bcl-2 gene can contribute to cancers where normal physiological cell death mechanisms are compromised by deregulation of the apoptotic influence of Bcl-2. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 26/22 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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