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Anti-ATP1A1 Antibody (7L590)
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产品编号 TMAC-03821
别名 Sodium/potassium-transporting ATPase subunit alpha-1, Sodium pump subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, EC 3.6.3.9, ATP1A1
Anti-ATP1A1 Antibody (7L590) 是一种 Rabbit 抗体，靶向 ATP1A1。Anti-ATP1A1 Antibody (7L590) 可用于 WB,ICC/IF,IHC,FCM。
Anti-ATP1A1 Antibody (7L590)
Anti-ATP1A1 Antibody (7L590)
还可以产品编号 TMAC-03821 别名 Sodium/potassium-transporting ATPase subunit alpha-1, Sodium pump subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, EC 3.6.3.9, ATP1A1
Anti-ATP1A1 Antibody (7L590) 是一种 Rabbit 抗体，靶向 ATP1A1。Anti-ATP1A1 Antibody (7L590) 可用于 WB,ICC/IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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| 产品描述 | Anti-ATP1A1 Antibody (7L590) is a Rabbit antibody targeting ATP1A1. Anti-ATP1A1 Antibody (7L590) can be used in WB,ICC/IF,IHC,FCM. |
| 别名 | Sodium/potassium-transporting ATPase subunit alpha-1, Sodium pump subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, EC 3.6.3.9, ATP1A1 |
| Ig Type | IgG |
| 克隆号 | 7L590 |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of Sodium Potassium ATPase on A549 cell lysates using anti-Sodium Potassium ATPase antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Sodium Potassium ATPase antibody. Counter stained with hematoxylin. 6. ICC staining Sodium Potassium ATPase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Sodium Potassium ATPase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with Sodium Potassium ATPase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The ubiquitously expressed sodium/potassium-ATPase (Na+/K+-ATPase) exists as an oligomeric plasma membrane complex that couples the hydrolysis of one molecule of ATP to the importation of three Na+ ions and two K+ ions against their respective electrochemical gradients. As a member of the P-type family of ion motives, Na+/K+-ATPase plays a critical role in maintaining cellular volume, resting membrane potential and Na+-coupled solute transport. Multiple isoforms of three subunits, α, β and γ, comprise the Na+/K+-ATPase oligomer. The α subunit contains the binding sites for ATP and the cations; the glycosylated β subunit ensures correct folding and membrane insertion of the α subunits. The small γ subunit co-localizes with the α subunit in nephron segments, where it increases the affinity of Na+/K+-ATPase for ATP. The β subunit, but not the γ subunit, is essential for normal activity of Na+/K+-ATPase. |
| 偶联 | Unconjugated |
| 免疫原 | A synthesized peptide: within Human ATP1A1 aa 39-83 / 1023 |
| 抗原种属 | human |
| Uniprot ID |
| 分子量 | Theoretical: 100 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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