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Anti-Annexin V/ANXA5 Antibody (2Q736) 是一种 Rabbit 抗体,靶向 Annexin V/ANXA5。Anti-Annexin V/ANXA5 Antibody (2Q736) 可用于 WB,ICC/IF,FCM。
Anti-Annexin V/ANXA5 Antibody (2Q736) 是一种 Rabbit 抗体,靶向 Annexin V/ANXA5。Anti-Annexin V/ANXA5 Antibody (2Q736) 可用于 WB,ICC/IF,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,485 | 5日内发货 |
产品描述 | Anti-Annexin V/ANXA5 Antibody (2Q736) is a Rabbit antibody targeting Annexin V/ANXA5. Anti-Annexin V/ANXA5 Antibody (2Q736) can be used in WB,ICC/IF,FCM. |
别名 | RPRGL3, PP4, HEL-S-7, ENX2, ANX5, annexin A5 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of Annexin V on different lysates using anti-Annexin V antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: HepG2, Lane 3: JAR. 2. ICC staining Annexin V in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining Annexin V in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. Flow cytometric analysis of Hela cells with Annexin V antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000; ICC/IF: 1:100-500; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | The annexin family of calcium-binding proteins is composed of at least ten mammalian genes and is characterized by a conserved core domain, which binds phospholipids in a Ca2+-dependent manner, and a unique amino-terminal region, which may confer binding specificity. Annexin family members have been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. For example, the crystal structure of Annexin III has suggested a hydrophilic amino-terminus with possible Ca2+ channel activity. Similarly, Annexin V has ion channel properties. Annexin IV, also referred to as endonexin, functions to regulate Cl-flux by mediating calmodulin kinase II (CaMKII) activity and Annexin V has been shown to regulate PKC activity. Annexin V is ubiquitously expressed at high levels in tissues and cells grown in tissue culture, while Annexin VIII exhibits a more limited distribution. Where co-expressed in the same tissues, Annexin VIII is often expressed at a 100-fold lower level than Annexin V. However, Annexin VIII is preferentially expressed in acute promyelocytic leukemia (APL) cells, which may relate to its role in hematopoietic cell differentiation. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 36 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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