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Anti-AMPK α1 Antibody (7H670)

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Anti-AMPK α1 Antibody (7H670)
产品编号 TMAC-00178
别名 wu:fa94c10, Tau protein kinase PRKAA1, SNF1-like protein AMPK, SNF1A, Protein kinase AMP activated alpha 1 catalytic subunit, PRKAA1, PRKAA 1, OTTHUMP00000161796, OTTHUMP00000161795, MGC57364, MGC33776, kinase AMPK alpha1, im:7154392, Hydroxymethylglutaryl CoA reductase kinase, hormone sensitive lipase kinase, HMGCR kinase, HMG CoA reductase kinase, EC 2.7.11.1, cb116, C130083N04Rik, AMPKα1, AMPKalpha1, AMPKa1, AMPK1, AMPK subunit alpha-1, AMPK alpha1, AMPK alpha 1 chain, AMPK alpha 1, AMPK a1, AMPK 1, AMPK, AMP-activated protein kinase, catalytic, alpha -1, AMP -activate kinase alpha 1 subunit, AL024255, AI450832, AI194361, acetyl CoA carboxylase kinase, ACACA kinase, AAPK1_HUMAN, AAPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, 5' AMP activated protein kinase catalytic subunit alpha 1, 5 AMP activated protein kinase catalytic alpha 1 chain, 5 AMP activated protein kinase alpha 1catalytic subunit

Anti-AMPK α1 Antibody (7H670) 是一种 Rabbit 抗体，靶向 AMPK α1。Anti-AMPK α1 Antibody (7H670) 可用于 WB,ICC/IF,IP,FCM。

Anti-AMPK α1 Antibody (7H670)

Anti-AMPK α1 Antibody (7H670)

Rating icon 还可以
Anti-AMPK α1 Antibody (7H670)
产品编号 TMAC-00178 别名 wu:fa94c10, Tau protein kinase PRKAA1, SNF1-like protein AMPK, SNF1A, Protein kinase AMP activated alpha 1 catalytic subunit, PRKAA1, PRKAA 1, OTTHUMP00000161796, OTTHUMP00000161795, MGC57364, MGC33776, kinase AMPK alpha1, im:7154392, Hydroxymethylglutaryl CoA reductase kinase, hormone sensitive lipase kinase, HMGCR kinase, HMG CoA reductase kinase, EC 2.7.11.1, cb116, C130083N04Rik, AMPKα1, AMPKalpha1, AMPKa1, AMPK1, AMPK subunit alpha-1, AMPK alpha1, AMPK alpha 1 chain, AMPK alpha 1, AMPK a1, AMPK 1, AMPK, AMP-activated protein kinase, catalytic, alpha -1, AMP -activate kinase alpha 1 subunit, AL024255, AI450832, AI194361, acetyl CoA carboxylase kinase, ACACA kinase, AAPK1_HUMAN, AAPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, 5' AMP activated protein kinase catalytic subunit alpha 1, 5 AMP activated protein kinase catalytic alpha 1 chain, 5 AMP activated protein kinase alpha 1catalytic subunit

Anti-AMPK α1 Antibody (7H670) 是一种 Rabbit 抗体，靶向 AMPK α1。Anti-AMPK α1 Antibody (7H670) 可用于 WB,ICC/IF,IP,FCM。

规格价格库存数量
50 μL
¥ 1,485
5日内发货
100 μL
¥ 2,480
5日内发货
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生物活性
产品描述
Anti-AMPK α1 Antibody (7H670) is a Rabbit antibody targeting AMPK α1. Anti-AMPK α1 Antibody (7H670) can be used in WB,ICC/IF,IP,FCM.
别名wu:fa94c10, Tau protein kinase PRKAA1, SNF1-like protein AMPK, SNF1A, Protein kinase AMP activated alpha 1 catalytic subunit, PRKAA1, PRKAA 1, OTTHUMP00000161796, OTTHUMP00000161795, MGC57364, MGC33776, kinase AMPK alpha1, im:7154392, Hydroxymethylglutaryl CoA reductase kinase, hormone sensitive lipase kinase, HMGCR kinase, HMG CoA reductase kinase, EC 2.7.11.1, cb116, C130083N04Rik, AMPKα1, AMPKalpha1, AMPKa1, AMPK1, AMPK subunit alpha-1, AMPK alpha1, AMPK alpha 1 chain, AMPK alpha 1, AMPK a1, AMPK 1, AMPK, AMP-activated protein kinase, catalytic, alpha -1, AMP -activate kinase alpha 1 subunit, AL024255, AI450832, AI194361, acetyl CoA carboxylase kinase, ACACA kinase, AAPK1_HUMAN, AAPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-1, 5' AMP activated protein kinase catalytic subunit alpha 1, 5 AMP activated protein kinase catalytic alpha 1 chain, 5 AMP activated protein kinase alpha 1catalytic subunit
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of AMPK alpha 1 on different lysates using anti-AMPK alpha 1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: HepG2, Lane 3: MCF-7.
2. ICC staining AMPK alpha 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. ICC staining AMPK alpha 1 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining AMPK alpha 1 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. Flow cytometric analysis of Hela cells with AMPK alpha 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
应用
WB
ICC/IF
IP
FCM
推荐剂量
WB: 1:1000-5000; ICC/IF: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex com-prising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 and AMPKα2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKβ1 encodes a 271 amino acid protein and human AMPKβ2 encodes a 272 amino acid protein. The human AMPKγ1 gene encodes a 331 amino acid protein. Human AMPKγ2 and AMPKγ3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
Q13131
化学信息
分子量Theoretical: 63 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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