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Anti-ALDH1A1 Antibody (6M553)
还可以产品编号 TMAY-01851
别名 RALDH1, PUMB1, HEL-S-53e, HEL-9, HEL12, ALDH-E1, ALDH11, ALDH1, aldehyde dehydrogenase 1 family, member A1, ALDC
Anti-ALDH1A1 Antibody (6M553) 是一种 Mouse 抗体，靶向 ALDH1A1。Anti-ALDH1A1 Antibody (6M553) 可用于 WB,ELISA,FCM,ICC/IF。
Anti-ALDH1A1 Antibody (6M553)
Anti-ALDH1A1 Antibody (6M553)
还可以产品编号 TMAY-01851 别名 RALDH1, PUMB1, HEL-S-53e, HEL-9, HEL12, ALDH-E1, ALDH11, ALDH1, aldehyde dehydrogenase 1 family, member A1, ALDC
Anti-ALDH1A1 Antibody (6M553) 是一种 Mouse 抗体，靶向 ALDH1A1。Anti-ALDH1A1 Antibody (6M553) 可用于 WB,ELISA,FCM,ICC/IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 20 μL | ¥ 795 | 5日内发货 | |
| 100 μL | ¥ 1,684 | 5日内发货 |
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偶联与修饰
抗原信息
| 产品描述 | Anti-ALDH1A1 Antibody (6M553) is a Mouse antibody targeting ALDH1A1. Anti-ALDH1A1 Antibody (6M553) can be used in WB,ELISA,FCM,ICC/IF. |
| 别名 | RALDH1, PUMB1, HEL-S-53e, HEL-9, HEL12, ALDH-E1, ALDH11, ALDH1, aldehyde dehydrogenase 1 family, member A1, ALDC |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 6M553 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human ALDH1A1 in MCF7 or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human ALDH1A1 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human ALDH1A1 on A549 cells. Cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human ALDH1A1, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells. 3. Anti-ALDH1A1 mouse monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:55 kDa. -Observed band size:55 kDa |
| 应用 | WB,ELISA,FCM,ICC/IF |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ALDH1 (rh ALDH1; TMPY-01583; NP_000680.2; Ser 2-Ser 501). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography . |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Aldehyde dehydrogenase 1 family, member A1 (ALDH1A1), also known as Aldehyde dehydrogenase 1 (ALDH1), or Retinaldehyde Dehydrogenase 1 (RALDH1), is an enzyme that is expressed at high levels in stem cells and that has been suggested to regulate stem cell function. The retinaldehyde dehydrogenase (RALDH) subfamily of ALDHs, composed of ALDH1A1, ALDH1A2, ALDH1A3, and ALDH8A1, regulate development by catalyzing retinoic acid biosynthesis. The ALDH1A1 protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. ALDH1A1 also belongs to the group of corneal crystallins that help maintain the transparency of the cornea. Increased ALDH1A1 activity has been found in the stem cell populations of leukemia and some solid tumors. In tumor specimens, increased ALDH1A1 immunopositivity was found not only in secretory type cancer epithelial cells but also in neuroendocrine tumor populations. ALDH1 has been identified as a reliable marker of breast cancer stem cells. ALDH1 expression in primary cancer is an independent prognostic factor in node-positive breast cancer patients. ALDH1A1 plays a key role in normal hematopoiesis, and as a TLX1 transcriptional target, ALDH1A1 may contribute to the ability of this homeoprotein to alter cell fate and induce tumor growth. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | FITC/PE |
| 可提供抗体种类 | 3 |
| 免疫原 | Recombinant Human ALDH1 protein (TMPY-01583) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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