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Anti-ADAM15 Antibody (5X82)
还可以产品编号 TMAY-00937
Anti-ADAM15 Antibody (5X82) 是一种 Rabbit 抗体，靶向 ADAM15。Anti-ADAM15 Antibody (5X82) 可用于 ELISA,ELISA(Det),IHC-P,FCM,ICC/IF。
Anti-ADAM15 Antibody (5X82)
Anti-ADAM15 Antibody (5X82)
还可以产品编号 TMAY-00937
Anti-ADAM15 Antibody (5X82) 是一种 Rabbit 抗体，靶向 ADAM15。Anti-ADAM15 Antibody (5X82) 可用于 ELISA,ELISA(Det),IHC-P,FCM,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-ADAM15 Antibody (5X82) is a Rabbit antibody targeting ADAM15. Anti-ADAM15 Antibody (5X82) can be used in ELISA,ELISA(Det),IHC-P,FCM,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 5X82 |
| 交叉反应 | Human |
| 验证活性 | 1. Profile of anti-ADAM15 reactivity on MCF-7 cells analyzed by flow cytometry. 2. Immunohistochemical staining of formalin fixed, paraffin-embedded human lymphonode showing membrane staining of endothelial cells (red arrow) (1:2000). 3. Immunohistochemical staining of formalin fixed, paraffin-embedded human heart showing blood vessel staining (red arrow) (1:200). 4. Immunofluorescence staining of Human ADAM15 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ADAM15 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm. |
| 应用 | ELISA,ELISA(Det),IHC-P,FCM,ICC/IF |
| 推荐剂量 | ELISA: 1:5000-1:10000; IHC-P: 1:1000-1:4000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100; ELISA(Det): 1:1000-1:10000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human ADAM15 (rh ADAM15; TMPY-00961; NP_997074.1; Met 1-Thr 696). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | ADAM15, also known as Metargidin, is a type I transmembrane glycoprotein belonging to the ADAM (A Disintegrin and Metalloprotease Domain) family of proteins and is widely expressed in different tissues and cell types. Members of this family contain an amino-terminal metalloprotease domain followed by a disintegrin domain, a cysteine-rich region and a membrane proximal EGF-like domain. The disintegrin domain of ADAM15/metargidin contains an RGD tripeptide sequence, suggesting that it may potentially interact with the integrin family of proteins. ADAM15 is a transmembrane multi-domain proteins implicated in proteolysis, cell-cell and cell-matrix interactions in various disease conditions. There is also evidence supporting a role for ADAM15 in angiogenesis and angioinvasion of tumor cells, which are critical for unrestrained tumor growth and metastatic spread. Given its diverse functions, ADAM15 may represent a pivotal regulatory component of tumor progression, an important target for therapeutic intervention, or emerge as a biomarker of disease progression. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | APC/PE |
| 可提供抗体种类 | 3 |
| 免疫原 | Recombinant Human ADAM15 protein (TMPY-00961) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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