Anti-ACTA2 Antibody (3G548) is a Rabbit antibody targeting ACTA2. Anti-ACTA2 Antibody (3G548) can be used in WB,ICC/IF,IHC,FCM.

IgG

Human,Mouse,Rat

1. Western blot analysis of alpha smooth muscle Actin on different lysates using anti-alpha smooth muscle Actin antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: A431, Lane 3: NIH/3T3.
2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
5. ICC staining alpha smooth muscle Actin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining alpha smooth muscle Actin in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining alpha smooth muscle Actin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining alpha smooth muscle Actin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. Flow cytometric analysis of Jurkat cells with alpha smooth muscle Actin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100

Monoclonal

Unconjugated

Recombinant Protein