Anti-ACP5 Polyclonal Antibody 2 is a Rabbit antibody targeting ACP5. Anti-ACP5 Polyclonal Antibody 2 can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Pig)

Blank control (black line): Hela.
Primary Antibody (green line): Rabbit Anti-TRAP5 antibody (TMAB-00040)
Dilution: 2 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM: 2ug/Test

Polyclonal

KLH conjugated synthetic peptide: human TRAP5

Human

Phosphatases,Phosphatases,Other Metabolism Assay