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Anti-4EBP1 Antibody (5U577)
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产品编号 TMAC-01276
别名 PHAS-I, eukaryotic translation initiation factor 4E binding protein 1, BP-1, 4E-BP1, 4EBP1
Anti-4EBP1 Antibody (5U577) 是一种 Rabbit 抗体，靶向 4EBP1。Anti-4EBP1 Antibody (5U577) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-4EBP1 Antibody (5U577)
Anti-4EBP1 Antibody (5U577)
还可以产品编号 TMAC-01276 别名 PHAS-I, eukaryotic translation initiation factor 4E binding protein 1, BP-1, 4E-BP1, 4EBP1
Anti-4EBP1 Antibody (5U577) 是一种 Rabbit 抗体，靶向 4EBP1。Anti-4EBP1 Antibody (5U577) 可用于 WB,ICC/IF,IHC,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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产品介绍
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偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-4EBP1 Antibody (5U577) is a Rabbit antibody targeting 4EBP1. Anti-4EBP1 Antibody (5U577) can be used in WB,ICC/IF,IHC,IP,FCM. |
| 别名 | PHAS-I, eukaryotic translation initiation factor 4E binding protein 1, BP-1, 4E-BP1, 4EBP1 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of eIF4EBP1 on different lysates using anti-eIF4EBP1 antibody at 1/1,000 dilution. Positive control: Lane 1: K562, Lane 2: HepG2, Lane 3: 293. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-eIF4EBP1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-eIF4EBP1 antibody. Counter stained with hematoxylin. 4. ICC staining eIF4EBP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining eIF4EBP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of NIH/3T3 cells with eIF4EBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The translation of proteins from eukaryotic mRNA is initiated by the multisubunit complex eIF-4F, which associates with the mRNA 5' cap structure. eIF-4E, a component of eIF-4F, is responsible for binding to the 5' cap structure and for the assembly of the eIF-4F complex. The regulatory protein 4E-BP1, also referred to as PHAS-I, inhibits eIF-4E function. Phosphorylation of 4E-BP1 by S6 kinase p70, MAP kinases or PKCs causes the disassociation of 4E-BP1 from eIF-4E, promoting translation. A protein that is functionally related to 4E-BP1, designated 4E-BP2, also associates with eIF-4E. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 17 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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