Powder: -20°C for 3 years | In solvent: -80°C for 2 years
(-)-Epicatechin 是一种 COX-1 的抑制剂，通过阻断 NF-Κb 的 p65 亚基的核定位来抑制 IL-1β 诱导的 iNOS 表达。
| 产品描述 | (-)-Epicatechin is an inhibitor of cyclooxygenase-1 (COX-1), inhibiting the IL-1β-induced expression of iNOS by blocking the nuclear localization of the p65 subunit of NF-κB. |
| 靶点活性 | COX-1:3.2 μM |
| 体外活性 | (-)-Epicatechin stimulates mitochondrial respiration and oxygen consumption in Panc-1 cells. Human normal fibroblasts are not affected. (-)-Epicatechin sensitizes Panc-1, U87, and MIA PaCa-2 cells with an average radiation enhancement factor (REF) of 1.7, 1.5, and 1.2, respectively. (-)-Epicatechin does not sensitize normal fibroblast cells to ionizing radiation with a REF of 0.9, suggesting cancer cell selectivity. (-)-Epicatechin enhances Chk2 phosphorylation and p21 induction when combined with radiation in cancer, but not normal cells[2]. |
| 体内活性 | Aging has deleterious effects on modulators of muscle growth/differentiation, the consumption of modest amounts of the flavanol (?)-epicatechin can partially reverse these changes. In mice, myostatin and senescence-associated β-galactosidase levels increase with aging, while those of follistatin and Myf5 decrease. (?)-Epicatechin decreases myostatin and β-galactosidase and increases levels of markers of muscle growth. In humans, myostatin and β-galactosidase increase with aging while follistatin, MyoD and myogenin decrease. Treatment for 7 days with (?)-epicatechin increases hand grip strength and the ratio of plasma follistatin/myostatin[1]. Low-dose (?)-epicatechin treatment resulted in significant increases in skeletal muscle capillarity, mitochondrial biogenesis, and oxidative enzyme activity, which corresponded to lower muscle fatigue and higher endurance capacity when compared to the placebo group. (?)-epicatechin may potentially regulate the protein expression between angiogenic and antiangiogenic factors, such as VEGF-A and TSP-1, respectively[3]. |
| 细胞实验 | Panc-1 cells are seeded into T-150 flasks and on the next day, cells are treated with different concentrations of (-)-epicatechin for 1 h, then harvested and solubilized in 10 mM HEPES (pH 7.4), 40 mM KCl, 1%Tween-20, 1 μM oligomycin, 1 mM PMSF,10 mM KF, 2 mM EGTA, and 1 mM Na3VO4. COX activity is measured in the presence of 20 mM ascorbate and 200 μM substrate cytochrome c from cow heart.(Only for Reference) |
| 别名 | 表儿茶素, (-)-Epicatechol, Epicatechin, L-Epicatechin, 表儿茶精 |
| 分子量 | 290.28 |
| 分子式 | C15H14O6 |
| CAS No. | 490-46-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 2 years
Ethanol: <1 mg/mL
H2O: <1 mg/mL
DMSO: 54 mg/mL (186 mM)
( < 1 mg/mL refers to the product slightly soluble or insoluble )
参考文献文献引用
剂量换算对于不同动物的给药剂量换算，您也可以参考 更多...
体内实验配液计算器请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法： 比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)， 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。
体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300， 混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。
计算器bottom
您可能有的问题的答案可以在抑制剂处理说明中找到，包括如何准备库存溶液，如何存储产品，以及基于细胞的分析和动物实验需要特别注意的问题。
(-)-Epicatechin 490-46-0 Apoptosis Cell Cycle/Checkpoint Immunology/Inflammation Metabolism Neuroscience COX Ferroptosis Endogenous Metabolite 表儿茶素 (-)-Epicatechol epi-Catechin Epicatechin Inhibitor L-Epicatechin inhibit ( ) Epicatechin Cyclooxygenase 表儿茶精 ()Epicatechin inhibitor
沪公网安备 31010602006700号
