rhod-ff

Rhod-FF is a difluorinated analog of the cell-impermeant calcium indicator rhod-2. It has a very low affinity for calcium (Kd = 320 μM). Low affinity calcium indicators are particularly useful for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes will be insensitive to luminal fluctuations. Rhod-FF has excitation/emission maxima of approximately 552 and 580 nm, respectively.

Rhod-FF AM是一种细胞渗透性的荧光钙离子指示剂，能够被细胞内酯酶水解生成rhod-FF，Ex/Em=552/580 nm。Rhod-FF AM与Ca²⁺的结合亲和力较低，可用于10-200 μM浓度范围的较高浓度钙离子检测。