rhod-2

Rhod-2 (sodium salt) is a water-soluble, red fluorescent calcium indicator. It exhibits a significant shift in fluorescence intensity upon calcium binding (ex max = 549 nm; calcium-free v. ex/em max = 552/581 nm; calcium-bound). [1][2] Unlike the UV-excitable indicators fura-2 and indo-1 , there is no accompanying spectral shift.

Rhod-2 (potassium salt) is a water-soluble, red fluorescent calcium indicator. It exhibits a significant shift in fluorescence intensity upon calcium binding (ex max = 549 nm; calcium-free v. ex/em max = 552/581 nm; calcium-bound). Unlike the UV-excitable indicators fura-2 and indo-1 , there is no accompanying spectral shift.

Rhod-2 AM 是一种线粒体荧光探针，λex 为 552 nm，λem 为 581 nm。

Rhod-2 (sodium) 是单波长Ca2+荧光染料(Kd: 570 nM) (Ex/Em: 557/581 nm)，用于研究细胞内Ca2+信号传导。

Rhod-FF tripotassium 是 Rhod-2 的衍生物，以及一种低亲和力的钙离子指示剂 (Kd为 320 μM)。该化合物与钙离子结合时会发生荧光变化，具有在 552 nm 激发和 580 nm 发射的特性，从而用以检测钙离子浓度。

Rhod-FF is a difluorinated analog of the cell-impermeant calcium indicator rhod-2. It has a very low affinity for calcium (Kd = 320 μM). Low affinity calcium indicators are particularly useful for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes will be insensitive to luminal fluctuations. Rhod-FF has excitation/emission maxima of approximately 552 and 580 nm, respectively.

Rhod-FF AM是一种细胞渗透性的荧光钙离子指示剂，能够被细胞内酯酶水解生成rhod-FF，Ex/Em=552/580 nm。Rhod-FF AM与Ca²⁺的结合亲和力较低，可用于10-200 μM浓度范围的较高浓度钙离子检测。