Phalloidin-AMCAconjugate is a blue fluorophore that specifically labels filamentous actin (F-actin). It consists of aminomethylcoumarin (AMCA; ex em max = 350 450 nm) conjugated with phalloidin , a mycotoxin that binds F-actin. Phalloidin-AMCAconjugate is used in fixed and permeabilized tissue sections, cell cultures, or cell-free experiments.