ac anw amc

Ac-ANW-AMC is a fluorogenic substrate for the β5i LMP7 subunit of the 20S immunoproteasome. [1] Upon cleavage, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify the activity of the β5i LMP7 subunit of the 20S immunoproteasome. AMC displays excitation emission maxima of 340-360 440-460 nm, respectively. Reference[1]. Winter, M.B., La Greca, F., Arastu-Kapur, S., et al. Immunoproteasome functions explained by divergence in cleavage specificity and regulation. eLife 6:e27364, (2017).