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VCAM-1 Protein, Mouse, Recombinant (His)

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货号 TMPY-01830

别名 Vcam-1, vascular cell adhesion molecule 1, CD106

VCAM-1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 75.8 kDa and the accession number is NP_035823.3.

VCAM-1 Protein, Mouse, Recombinant (His)

VCAM-1 Protein, Mouse, Recombinant (His)

一键复制产品信息
Rating icon 很棒
SPR兼容缓冲液
货号 TMPY-01830 别名 Vcam-1, vascular cell adhesion molecule 1, CD106

VCAM-1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 75.8 kDa and the accession number is NP_035823.3.

规格价格库存数量
5 μg
¥ 365
现货
10 μg
¥ 585
现货
20 μg
¥ 958
3日内发货
50 μg
¥ 1,880
3日内发货
100 μg
¥ 3,170
现货
200 μg
¥ 5,610
5日内发货
500 μg
¥ 11,900
5日内发货
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产品介绍


生物活性
产品描述
VCAM-1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 75.8 kDa and the accession number is NP_035823.3.
生物活性
Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to mouse VCAM1 coated plates (10 μg/ml with 100 μl/well), approximately 70%-80% cells will adhere after 1 hour at RT.
研究背景
Tumor necrosis factor alpha (TNFα) is a pro-inflammatory cytokine that triggers the expression of inflammatory molecules, including other cytokines and cell adhesion molecules. TNFα induces the expression of intercellular cell adhesion molecule-1 and vascular cell adhesion molecule-1 (VCAM-1). VCAM-1 was originally identified as a cell adhesion molecule that helps regulate inflammation-associated vascular adhesion and the transendothelial migration of leukocytes, such as macrophages and T cells.
种属
Mouse
表达系统
HEK293 Cells
标签C-His
蛋白编号NP_035823.3
蛋白构建Phe25-Glu698
蛋白纯度
> 95% as determined by SDS-PAGE
VCAM-1 Protein, Mouse, Recombinant (His)
蛋白性状Lyophilized powder
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
别名Vcam-1, vascular cell adhesion molecule 1, CD106
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
化学信息
分子量75.8 kDa (predicted); 80-100 kDa (reducing condition, due to glycosylation)
颜色White
物理性状Lyophilized powder
储存&溶解度
运输方式In general, Lyophilized powders are shipping with blue ice.
存储It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

计算器

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  • 重组蛋白稀释 计算器
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剂量转换

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技术支持

请阅读 重组蛋白用户指南 了解更多具体信息.

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