5T4/TPBG Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with C-mFc tag. The predicted molecular weight is 80-110 KDa and the accession number is Q13641.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 μg | ¥ 830 | 5日内发货 | ||
50 μg | ¥ 2,510 | 5日内发货 | ||
500 μg | ¥ 12,600 | 5日内发货 | ||
1 mg | ¥ 18,900 | 5日内发货 |
生物活性 | 1. Loaded Human TPBG Antibody (5G9) (Human IgG1) on Protein-A Biosensor, can bind Human TPBG (C-mFc) with an affinity constant of 8.75 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Human TPBG (C-mFc) at 2μg/ml (100 μl/well) can bind Human TPBG Antibody (5G9) (Human IgG1) . The ED50 of Human TPBG Antibody (5G9) (Human IgG1) is 1.57 ng/ml. (Regularly tested) |
产品描述 | 5T4/TPBG Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with C-mFc tag. The predicted molecular weight is 80-110 KDa and the accession number is Q13641. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-mFc |
蛋白编号 | Q13641 |
别名 | TPBG, 5T4 Protein, M6P1 Protein, 5T4AG Protein, WAIF1 Protein |
氨基酸序列 | Ser32-Ser355 |
蛋白构建 | Ser32-Ser355 |
蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 80-110 KDa (reducing condition) |
内毒素 | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
研究背景 | Trophoblast glycoprotein, also known as TPBG and 5T4, it belongs to the LRR family of proteins. The C-terminus of LRR N-terminal cap (LRRNT) and LRR 1 are essential for the inhibition of the Wnt signaling pathway. TPBG may function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. It expressed by all types of trophoblasts as early as 9 weeks of development. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma. |
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5T4/TPBG Protein, Human, Recombinant (mFc) TPBG 5T4 Protein M6P1 Protein 5T4AG Protein WAIF1 Protein recombinant recombinant-proteins proteins protein