TMP21 Protein, Human, Recombinant (mFc)

产品编号 TMPY-00499

TMED10 disrupts the complex formation between TGF-beta type I (also termed ALK5) and type II receptors (TbetaRII). Misexpression studies revealed that TMED10 attenuated TGF-beta-mediated signaling. A 20-amino acid-long region from Thr(91) to Glu(110) within the extracellular region of TMED10 was found to be crucial for TMED10 interaction with both ALK5 and TbetaRII. Synthetic peptides corresponding to this region inhibit both TGF-beta-induced Smad2 phosphorylation and Smad-dependent transcriptional reporter activity. In a xenograft cancer model, where previously TGF-beta was shown to elicit tumor-promoting effects, gain-of-function and loss-of-function studies for TMED10 revealed a decrease and increase in the tumor size, respectively. That TMED10 expression levels are the key determinant for efficiency of TGF-beta receptor complex formation and signaling.

TMP21 Protein, Human, Recombinant (mFc)

产品编号 TMPY-00499

规格	价格	库存	数量
100 μg	¥ 4,460	5日内发货

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常见问题解答

问超声一般需要多久？

答超声时长和溶解的量和浓度都有关，一般建议半个小时左右，如果浓度接近最大溶解度，或者需溶解的粉末较多，建议延长超声时间，并辅以加热。

问超声应使用什么仪器？

答建议使用“超声清洗仪”，不推荐用“超声破碎仪”。

问如何稀释工作液？

答根据您的工作液浓度，计算母液所需要稀释的倍数和所需母液的体积。建议用水、生理盐水、PBS等溶剂进行稀释，如果是细胞实验，可以使用培养基。取适当的溶剂缓慢加入到母液中，直到达到您的工作液浓度，可以通过涡旋或者移液枪轻轻吹打帮助混匀。我们的化合物大部分是脂溶性的，所以在使用 PBS 或者培养基等稀释工作液的时候，会有沉淀析出，这些都是正常现象，大多时候可以通过超声完全溶解。工作液建议现配现用！

问如何设置母液浓度？

答母液浓度需低于官网给出的溶解度，在这个范围内，根据工作液浓度设置母液浓度，细胞实验中，建议母液浓度设定在工作液浓度的 1000 倍以上。

问收到化合物后，是否需要称量后配制储备液？

答对于小包装，如 1 mg、5 mg、10 mg 等，不建议二次称量。这样会导致化合物损耗，且无法估算。TargetMol 所有产品原装进口，精准称量，您收到后可直接添加相应量溶剂。对于大包装，如果您需要分批次实验，可每次称量后配制储备液，建议您 10 mg 起称，称量的误差会小一些；当然条件允许，也可以先配制成高浓度母液，分装备用。

产品信息

生物活性	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述	TMED10 disrupts the complex formation between TGF-beta type I (also termed ALK5) and type II receptors (TbetaRII). Misexpression studies revealed that TMED10 attenuated TGF-beta-mediated signaling. A 20-amino acid-long region from Thr(91) to Glu(110) within the extracellular region of TMED10 was found to be crucial for TMED10 interaction with both ALK5 and TbetaRII. Synthetic peptides corresponding to this region inhibit both TGF-beta-induced Smad2 phosphorylation and Smad-dependent transcriptional reporter activity. In a xenograft cancer model, where previously TGF-beta was shown to elicit tumor-promoting effects, gain-of-function and loss-of-function studies for TMED10 revealed a decrease and increase in the tumor size, respectively. That TMED10 expression levels are the key determinant for efficiency of TGF-beta receptor complex formation and signaling.
种属	Human
表达系统	HEK293 Cells
标签	C-mFc
蛋白编号	P49755
别名	transmembrane emp24-like trafficking protein 10 (yeast),Tmp-21-I,TMP21,S31III125,S31I125,P24(δ),P24(DELTA),p23
蛋白构建	A DNA sequence encoding the human TMED10 (NP_006818.3) (Met1-Arg185) was expressed with the Fc region of mouse IgG1 at the C-terminus. Predicted N terminal: Ile 32
蛋白纯度	> 85 % as determined by SDS-PAGE
分子量	44 kDa (predicted)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	TMED10 disrupts the complex formation between TGF-beta type I (also termed ALK5) and type II receptors (TbetaRII). Misexpression studies revealed that TMED10 attenuated TGF-beta-mediated signaling. A 20-amino acid-long region from Thr(91) to Glu(110) within the extracellular region of TMED10 was found to be crucial for TMED10 interaction with both ALK5 and TbetaRII. Synthetic peptides corresponding to this region inhibit both TGF-beta-induced Smad2 phosphorylation and Smad-dependent transcriptional reporter activity. In a xenograft cancer model, where previously TGF-beta was shown to elicit tumor-promoting effects, gain-of-function and loss-of-function studies for TMED10 revealed a decrease and increase in the tumor size, respectively. That TMED10 expression levels are the key determinant for efficiency of TGF-beta receptor complex formation and signaling.

TMP21 Protein, Human, Recombinant (mFc)

TMP21 Protein, Human, Recombinant (mFc)

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