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顶部产品介绍化学信息储存和溶解信息计算器技术支持

SPARC Protein, Human, Recombinant (His)

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货号 TMPY-01182

别名 SPARC, secreted protein, acidic, cysteine-rich (osteonectin), ON, BM-40

SPARC Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 33.8 kDa and the accession number is P09486.

SPARC Protein, Human, Recombinant (His)
其他形式的 “SPARC Protein, Human, Recombinant (His)”:
SPARC Protein, Cynomolgus, Recombinant (His)
SPARC Protein, Mouse, Recombinant (His)
SPARC Protein, Rat, Recombinant (His)

SPARC Protein, Human, Recombinant (His)

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Rating icon 还可以
SPR兼容缓冲液
货号 TMPY-01182 别名 SPARC, secreted protein, acidic, cysteine-rich (osteonectin), ON, BM-40

SPARC Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 33.8 kDa and the accession number is P09486.

规格价格库存数量
5 μg
¥ 428
6-8日内发货
10 μg
¥ 683
6-8日内发货
20 μg
¥ 1,130
5日内发货
50 μg
¥ 2,270
5日内发货
100 μg
¥ 4,430
5日内发货
200 μg
¥ 6,730
5日内发货
500 μg
¥ 13,800
5日内发货
1 mg
¥ 28,800
5日内发货
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产品介绍

生物活性
产品描述
SPARC Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 33.8 kDa and the accession number is P09486.
生物活性
Immobilized Human SPARC, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-SPARC Antibody, hFc Tag with the EC50 of 0.10 μg/ml determined by ELISA.
研究背景
Secreted protein acidic and rich in cysteine (SPARC/osteonectin/BM40) is one of the most abundant non-collagenous protein expressed in mineralized tissues.The capacity of SPARC to influence pathways involved in extracellular matrix assembly such as procollagen processing and collagen fibril formation as well as the capacity to influence osteoblast differentiation and osteoclast activity will be addressed.
种属
Human
表达系统
HEK293 Cells
标签C-His
蛋白编号P09486
蛋白构建Ala18-Ile303
蛋白纯度
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
蛋白性状Lyophilized powder
缓冲液Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
别名SPARC, secreted protein, acidic, cysteine-rich (osteonectin), ON, BM-40
内毒素Less than 1EU per μg by the LAL method.
化学信息
分子量33.8 kDa (Predicted); 40-50 kDa (Due to glycosylation)
储存&溶解度
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
存储It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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关键词

secreted protein, acidic, cysteine-richosteonectinBM40BM 40
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