SIRP alpha V2 Protein, Human, Recombinant (hFc)

产品编号 TMPK-00610

Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction. SIRP alpha V2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 63.9 kDa and the accession number is AAH26692.1.

SIRP alpha V2 Protein, Human, Recombinant (hFc)

产品编号 TMPK-00610

规格	价格	库存
100 μg	¥ 2,660	5日内发货
500 μg	¥ 10,600	5日内发货
1 mg	¥ 17,800	5日内发货

大包装 & 定制

加入购物车

TargetMol 的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。

实验操作小课堂

常见问题解答

问重组蛋白有吗？

答您需要什么种属的蛋白呢，对表达系统和标签有要求吗，用于什么实验？

问重组蛋白产品是如何运输的？

答大部分重组蛋白产品为冻⼲粉，对温度不敏感，在室温下稳定，一般室温或蓝冰运输。对于液体类蛋白，一般采用⼲冰运输。如果您收到产品时发现冰盒已经融化，无需担心，蛋白可以正常使用，短时间内不会影响产品质量。如果特定蛋白在室温下不稳定，我们会提供相应的低温运输方式，以确保产品质量不受影响。

问如何将冻干的重组蛋白配置为储备液？

答在打开盖⼦之前，先使用 10000-12000 rpm 的转速进行离心，20-30 秒，将附着在管盖或管壁上的冻⼲粉收集到管底，以避免损失。大多数蛋白可通过加入无菌蒸馏水来重新溶解。如果需要除水以外的其他稀释剂，产品 COA 中会对其进行说明。一般储备液浓度建议不要低于 100 μg/mL，分装储存避免反复冻融。

问为什么某些重组蛋白溶液中还需要添加载体蛋白？

答载体蛋白通常被用于提高蛋白的稳定性，防止蛋白在冷冻或解冻过程中粘附在管壁上。塑料管对蛋白有一定的吸附能力，这可能导致蛋白和管壁之间难以分离，导致溶液中蛋白的实际浓度下降，从而影响其活性。为减少这种损失，在长期保存重组蛋白产品之前，建议添加常用的载体蛋白溶液，例如0.1% BSA（牛血清白蛋白）、5% HSA（人血清白蛋白）、10% FBS（胎牛血清）或 5% 海藻糖等。如果重组蛋白的浓度较低，强烈建议添加适量的载体蛋白。

问为什么重组蛋白的体积和质量与预期不符？

答蛋白冻干粉由蛋白原液冻干而来，冻干粉质量不等同于蛋白质量。由于冻⼲产品的体积大⼩受多种因素影响，包括冻⼲前缓冲液的组分、盐离⼦浓度以及产品本身的浓度等。冻⼲粉可能看上去很多，也可能肉眼不易观察到，这些都是正常现象，请您放心使用。在收到产品后，照常复溶、使用或分装储存即可。

产品信息

生物活性	Immobilized Human SIRP alpha V2, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 33.9ng/ml determined by ELISA.
产品描述	Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction. SIRP alpha V2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 63.9 kDa and the accession number is AAH26692.1.
种属	Human
表达系统	HEK293 Cells
标签	C-hFc
蛋白编号	AAH26692.1
别名	SIRPα2,SIRPalpha2,SIRPA,SIRP α V2/CD172a,SIRP α,SIRP alpha,SHPS-1,SHPS1,PTPNS1,P84,MYD-1,MYD1,MFR,CD172a,BIT
蛋白构建	Glu31-Arg369
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	63.9 kDa (predicted). Due to glycosylation, the protein migrates to 75-85 kDa based on Tris-Bis PAGE result.
内毒素	< 1 EU/μg by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.

SIRP alpha V2 Protein, Human, Recombinant (hFc)

SIRP alpha V2 Protein, Human, Recombinant (hFc)

产品信息

参考文献

SCI 文献

计算器

技术支持

关键词