KIRREL3 重组蛋白, Rat (His)

KIRREL3 Protein, Rat, Recombinant (His)

产品编号 TMPY-03241

KIRREL3 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 56.4 kDa and the accession number is Q09GS6.

规格	价格	库存	数量
100 μg	¥ 3,820	5日内发货

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常见问题解答

问重组蛋白有吗？

答您需要什么种属的蛋白呢，对表达系统和标签有要求吗，用于什么实验？

问重组蛋白产品是如何运输的？

答大部分重组蛋白产品为冻⼲粉，对温度不敏感，在室温下稳定，一般室温或蓝冰运输。对于液体类蛋白，一般采用⼲冰运输。如果您收到产品时发现冰盒已经融化，无需担心，蛋白可以正常使用，短时间内不会影响产品质量。如果特定蛋白在室温下不稳定，我们会提供相应的低温运输方式，以确保产品质量不受影响。

问为什么某些重组蛋白溶液中还需要添加载体蛋白？

答载体蛋白通常被用于提高蛋白的稳定性，防止蛋白在冷冻或解冻过程中粘附在管壁上。塑料管对蛋白有一定的吸附能力，这可能导致蛋白和管壁之间难以分离，导致溶液中蛋白的实际浓度下降，从而影响其活性。为减少这种损失，在长期保存重组蛋白产品之前，建议添加常用的载体蛋白溶液，例如0.1% BSA（牛血清白蛋白）、5% HSA（人血清白蛋白）、10% FBS（胎牛血清）或 5% 海藻糖等。如果重组蛋白的浓度较低，强烈建议添加适量的载体蛋白。

问蛋白能保证活性吗？

答我们有现货的产品，有活性数据的话会展示在官网生物活性那栏，没有数据的也不代表就一定没有活性，是我们还没有建立活性检测方法，没有进行测试，但是我们的蛋白都是优先按照有活性的方法去表达纯化的。

问如何将冻干的重组蛋白配置为储备液？

答在打开盖⼦之前，先使用 10000-12000 rpm 的转速进行离心，20-30 秒，将附着在管盖或管壁上的冻⼲粉收集到管底，以避免损失。大多数蛋白可通过加入无菌蒸馏水来重新溶解。如果需要除水以外的其他稀释剂，产品 COA 中会对其进行说明。一般储备液浓度建议不要低于 100 μg/mL，分装储存避免反复冻融。

产品信息

生物活性	Measured by the ability of the immobilized protein to support the adhesion of MS1 mouse pancreatic islet endothelial cells. When cells are added to rat KIRREL3 coated plates (15 μg/mL, 100 μL/well), > 20% will adhere specifically after 90 minutes at 37 ℃.
产品描述	KIRREL3 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 56.4 kDa and the accession number is Q09GS6.
种属	Rat
表达系统	HEK293 Cells
标签	C-His
蛋白编号	Q09GS6
别名	kin of IRRE like 3 (Drosophila)
蛋白构建	A DNA sequence encoding the rat KIRREL3 (Q09GS6) (Met1-Ala523) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Leu 22
蛋白纯度	> 95 % as determined by SDS-PAGE
分子量	56.4 kDa (predicted); 60-64 kDa (reducing condition, due to glycosylation)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Kin of IRRE-like protein 3 (KIRREL3) also known as nin of irregular chiasm-like protein 3 or nephrin-like protein 2 (NEPH2) is a member of the nephrin-like protein family of transmembrane proteins, which includes NEPH1 (KIRREL) and NEPH3 (KIRREL2). KIRREL3/NEPH2 is expressed in the fetal and adult brain, and also in podocytes of kidney glomeruli. The cytoplasmic domains of KIRREL3/NEPH2 interact with the C-terminus of podocin, also expressed in the podocytes, cells involved in ensuring size- and charge-selective ultrafiltration. Mutations in KIRREL3/NEPH2 are associated with mental retardation autosomal dominant type 4. KIRREL3/NEPH2 expression is turned on in migrating Nucleogenesis of the pontine nucleus (PN) neurons only after they enter the presumptive nuclear region. KIRREL3/NEPH2 knockdown disrupted the nuclear organization of PN presumably by changing the migratory behavior of PN neurons inside the nuclear region. Moreover, overexpression of the cytoplasmic region of KIRREL3, which can sequester intracellular signaling of endogenous KIRREL3, resulted in similar phenotypes. Overall, these results suggest KIRREL3 is involved in the Nucleogenesis of the PN through the control of neuronal migration inside the nucleus.

KIRREL3 Protein, Rat, Recombinant (His)

KIRREL3 Protein, Rat, Recombinant (His)

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