ISG15 Protein, Human, Recombinant (His)

Ubiquitin-Like Protein ISG15 (ISG15) is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha and -beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. ISG15 becomes conjugated to a diverse set of proteins after IFN-alpha/beta stimulation or microbial challenge. The functions or biochemical consequences ISG15 conjugation to proteins are not yet known, but it appears that this modification does not target proteins for proteasomal degradation. ISG15 shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. Upon interferon treatment, ISG15 can be detected in both free and conjugated forms, and is secreted from monocytes and lymphocytes where it can function as a cytokine. In the cell, ISG15 co-localizes with intermediate filaments and ISGylation may modulate the JAK-STAT pathway or certain aspects of neurological disease.

规格	价格/CNY		数量
10 μg	￥ 100	5日内发货
50 μg	￥ 340	5日内发货
500 μg	￥ 1,070	5日内发货
1 mg	￥ 1,570	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 100

5日内发货

50 μg

￥ 340

5日内发货

500 μg

￥ 1,070

5日内发货

1 mg

￥ 1,570

5日内发货

产品描述

种属	Human
表达系统	E. coli
标签	C-6xHis
蛋白编号	AAH09507.1
别名	ISG15, Ubiquitin Cross-Reactive Protein, G1P2, Interferon-Induced 15 kDa Protein, Ubiquitin-Like Protein ISG15, Interferon-Induced 17 kDa Protein, UCRP, hUCRP, IP17
氨基酸序列	Gly2-Gly157
蛋白构建	Gly2-Gly157
蛋白纯度	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量	16 KDa (reducing condition)

内毒素	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 100 mM NaCl, pH 8.0.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	Ubiquitin-Like Protein ISG15 (ISG15) is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha and -beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. ISG15 becomes conjugated to a diverse set of proteins after IFN-alpha/beta stimulation or microbial challenge. The functions or biochemical consequences ISG15 conjugation to proteins are not yet known, but it appears that this modification does not target proteins for proteasomal degradation. ISG15 shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. Upon interferon treatment, ISG15 can be detected in both free and conjugated forms, and is secreted from monocytes and lymphocytes where it can function as a cytokine. In the cell, ISG15 co-localizes with intermediate filaments and ISGylation may modulate the JAK-STAT pathway or certain aspects of neurological disease.

计算器

复溶计算器

重组蛋白稀释计算器

比活力计算器

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

bottom

ISG15 Protein, Human, Recombinant (His)

计算器

技术支持

Keywords