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FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated

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货号 TMPY-05650

别名 Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha

FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 45.3 kDa and the accession number is Q8SPV9&Q8SPW0.

FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated

FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated

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SPR兼容缓冲液
货号 TMPY-05650 别名 Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha

FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 45.3 kDa and the accession number is Q8SPV9&Q8SPW0.

规格价格库存数量
5 μg
¥ 987
3日内发货
10 μg
¥ 1,730
3日内发货
20 μg
¥ 2,930
现货
50 μg
¥ 4,730
5日内发货
100 μg
¥ 6,830
5日内发货
200 μg
¥ 11,600
5日内发货
500 μg
¥ 23,500
5日内发货
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纯度: SDS-PAGE: 98.3%; SEC-HPLC: 91.3%
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产品介绍


生物活性
产品描述
FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 45.3 kDa and the accession number is Q8SPV9&Q8SPW0.
生物活性
1. Immobilized Anti-CD20 Antibody at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus FCGRT&B2M Heterodimer Protein (His & AVI Tag), Biotinylated, the EC50 is 80-240 ng/mL.
_x000D_ 2. Loaded Biotinylated Cynomolgus FcRn recombinant protein ( His & Avi Tag ) on SA Biosensor, can bind IgG1 Fc with an affinity constant of 0.08 μM as determined in a BLI assay.
_x000D_ 3. The purity of Cynomolgus FCGRT & B2M Heterodimer Protein (His & AVI Tag), Biotinylated is more than 90% and the molecular weight of this protein is around 46-56 kDa verified by SEC-MALS.
FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), BiotinylatedFCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), BiotinylatedFCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated
种属
Cynomolgus
表达系统
HEK293 Cells
标签His, Avi
蛋白编号Q8SPV9&Q8SPW0
蛋白构建A DNA sequence encoding the cynomolgus FCGRT (Q8SPV9) (Met 1-Ser 297) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the cynomolgus B2M (Q8SPW0) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Ala 24 & Ile 21
蛋白纯度
> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), BiotinylatedFCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated
蛋白性状Lyophilized powder
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
别名Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
化学信息
分子量45.3 kDa (predicted)
储存&溶解度
运输方式In general, Lyophilized powders are shipping with blue ice.
存储It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

计算器

  • 复溶 计算器
  • 重组蛋白稀释 计算器
  • 比活力 计算器

剂量转换

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技术支持

请阅读 重组蛋白用户指南 了解更多具体信息.
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