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FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated
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货号 TMPY-05650
别名 Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha
FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 45.3 kDa and the accession number is Q8SPV9&Q8SPW0.
其他形式的 “FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated”:
FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated
一键复制产品信息 很棒SPR兼容缓冲液
货号 TMPY-05650 别名 Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha
FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 45.3 kDa and the accession number is Q8SPV9&Q8SPW0.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 5 μg | ¥ 987 | 3日内发货 | |
| 10 μg | ¥ 1,730 | 3日内发货 | |
| 20 μg | ¥ 2,930 | 现货 | |
| 50 μg | ¥ 4,730 | 5日内发货 | |
| 100 μg | ¥ 6,830 | 5日内发货 | |
| 200 μg | ¥ 11,600 | 5日内发货 | |
| 500 μg | ¥ 23,500 | 5日内发货 |
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纯度: SDS-PAGE: 98.3%; SEC-HPLC: 91.3%
颜色: 白色
性状: Lyophilized powder
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产品介绍
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| 产品描述 | FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 45.3 kDa and the accession number is Q8SPV9&Q8SPW0. |
| 生物活性 | 1. Immobilized Anti-CD20 Antibody at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus FCGRT&B2M Heterodimer Protein (His & AVI Tag), Biotinylated, the EC50 is 80-240 ng/mL. _x000D_ 2. Loaded Biotinylated Cynomolgus FcRn recombinant protein ( His & Avi Tag ) on SA Biosensor, can bind IgG1 Fc with an affinity constant of 0.08 μM as determined in a BLI assay. _x000D_ 3. The purity of Cynomolgus FCGRT & B2M Heterodimer Protein (His & AVI Tag), Biotinylated is more than 90% and the molecular weight of this protein is around 46-56 kDa verified by SEC-MALS.    |
| 种属 | Cynomolgus |
| 表达系统 | HEK293 Cells |
| 标签 | His, Avi |
| 蛋白编号 | Q8SPV9&Q8SPW0 |
| 蛋白构建 | A DNA sequence encoding the cynomolgus FCGRT (Q8SPV9) (Met 1-Ser 297) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the cynomolgus B2M (Q8SPW0) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Ala 24 & Ile 21 |
| 蛋白纯度 | > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC   |
| 蛋白性状 | Lyophilized powder |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| 复溶方法 | Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot. |
| 别名 | Fc fragment of IgG, receptor, transporter, α, Fc fragment of IgG, receptor, transporter, alpha |
| 内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
| 分子量 | 45.3 kDa (predicted) |
| 运输方式 | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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