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ERG26 Protein, S. cerevisiae, Recombinant (His & Myc)

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货号 TMPH-03460

别名 Sterol-4-alpha-carboxylate 3-dehydrogenase ERG26, decarboxylating, Ergosterol biosynthetic protein 26, ERG26, decarboxylating, C-4 decarboxylase ERG26, C-3 sterol dehydrogenase ERG26

Essential enzyme in the ergosterol biosynthetic pathway. Part of a 3 enzyme system that catalyzes the sequential removal of the 2 methyl groups at the sterol C-4 position, converting 4,4-dimethylzymosterol to zymosterol. Catalyzes the second step, an oxidative decarboxylation that results in a reduction of the 3-beta-hydroxy group at the C-3 carbon to an oxo group. ERG26 Protein, S. cerevisiae, Recombinant (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 46.1 kDa and the accession number is P53199.

ERG26 Protein, S. cerevisiae, Recombinant (His & Myc)
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ERG26 Protein, S. cerevisiae, Recombinant (His & Myc)

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货号 TMPH-03460 别名 Sterol-4-alpha-carboxylate 3-dehydrogenase ERG26, decarboxylating, Ergosterol biosynthetic protein 26, ERG26, decarboxylating, C-4 decarboxylase ERG26, C-3 sterol dehydrogenase ERG26

Essential enzyme in the ergosterol biosynthetic pathway. Part of a 3 enzyme system that catalyzes the sequential removal of the 2 methyl groups at the sterol C-4 position, converting 4,4-dimethylzymosterol to zymosterol. Catalyzes the second step, an oxidative decarboxylation that results in a reduction of the 3-beta-hydroxy group at the C-3 carbon to an oxo group. ERG26 Protein, S. cerevisiae, Recombinant (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 46.1 kDa and the accession number is P53199.

规格价格库存数量
5 μg
¥ 825
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10 μg
¥ 1,360
20日内发货
20 μg
¥ 2,290
20日内发货
50 μg
¥ 3,460
20日内发货
100 μg
¥ 4,750
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200 μg
¥ 6,830
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500 μg
¥ 10,800
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1 mg
¥ 16,000
20日内发货
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产品介绍


生物活性
产品描述
Essential enzyme in the ergosterol biosynthetic pathway. Part of a 3 enzyme system that catalyzes the sequential removal of the 2 methyl groups at the sterol C-4 position, converting 4,4-dimethylzymosterol to zymosterol. Catalyzes the second step, an oxidative decarboxylation that results in a reduction of the 3-beta-hydroxy group at the C-3 carbon to an oxo group. ERG26 Protein, S. cerevisiae, Recombinant (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 46.1 kDa and the accession number is P53199.
生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
研究背景
Essential enzyme in the ergosterol biosynthetic pathway. Part of a 3 enzyme system that catalyzes the sequential removal of the 2 methyl groups at the sterol C-4 position, converting 4,4-dimethylzymosterol to zymosterol. Catalyzes the second step, an oxidative decarboxylation that results in a reduction of the 3-beta-hydroxy group at the C-3 carbon to an oxo group.
种属
SVG-Saccharomyces cerevisiae
表达系统
E. coli
标签N-10xHis, C-Myc
蛋白编号P53199
氨基酸序列MSKIDSVLIIGGSGFLGLHLIQQFFDINPKPDIHIFDVRDLPEKLSKQFTFNVDDIKFHKGDLTSPDDMENAINESKANVVVHCASPMHGQNPDIYDIVNVKGTRNVIDMCKKCGVNILVYTSSAGVIFNGQDVHNADETWPIPEVPMDAYNETKAIAEDMVLKANDPSSDFYTVALRPAGIFGPGDRQLVPGLRQVAKLGQSKFQIGDNNNLFDWTYAGNVADAHVLAAQKLLDPKTRTAVSGETFFITNDTPTYFWALARTVWKADGHIDKHVIVLKRPVAICAGYLSEWVSKMLGKEPGLTPFRVKIVCAYRYHNIAKAKKLLGYTPRVGIEEGINKTLAWMDEGL
蛋白构建1-349 aa
蛋白纯度
> 90% as determined by SDS-PAGE.
缓冲液If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
复溶方法Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
别名Sterol-4-alpha-carboxylate 3-dehydrogenase ERG26, decarboxylating, Ergosterol biosynthetic protein 26, ERG26, decarboxylating, C-4 decarboxylase ERG26, C-3 sterol dehydrogenase ERG26
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
化学信息
分子量46.1 kDa (predicted)
储存&溶解度
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
存储Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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