ErbB4 重组蛋白, Mouse (His)

ErbB4 Protein, Mouse, Recombinant (His)

产品编号 TMPY-03021

ErbB4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 71.5 kDa and the accession number is Q99P91.

规格	价格	库存	数量
100 μg	¥ 3,820	5日内发货

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ErbB4 Protein, Mouse, Recombinant (His)产品问题解答

问请问 IL-4 Protein, Mouse, Recombinant 应怎么保存以及用什么复溶？

答无菌水，浓度不低于100 μg/mL，且每管的体积不大于20 μL 进行保存。

问请问 IL-4 Protein, Mouse, Recombinant 可以用溶剂5%海藻糖溶解吗？

答IL-4 Protein, Mouse, Recombinant 用无菌去离子水复溶就行，建议浓度是0.25 mg/mL，最低不要低于0.1 mg/mL分装储存稀释根据实验体系用培养基稀释，如果担心蛋白储存稳定性的也可以用5%海藻糖溶解。

问请问 IL-4 Protein, Mouse, Recombinant 可以用于巨噬细胞极化吗？

答IL-4是一种由活化的T辅助细胞2 (Th2) 分泌的细胞因子，它主要诱导巨噬细胞向M2型极化。IL-4的使用剂量通常为10-100 ng/mL。例如，在一项研究中，使用20 ng/mL的IL-4来诱导 RAW 264.7 巨噬细胞向 M2 型极化，参考文献：The removal of 1,4-dioxane from polyester manufacturing process wastewater using an up-flow Biological Aerated Filter (UBAF) packed with tire chips；因此可以用于巨噬细胞极化。

常见问题解答

问如何将冻干的重组蛋白配置为储备液？

答在打开盖⼦之前，先使用 10000-12000 rpm 的转速进行离心，20-30 秒，将附着在管盖或管壁上的冻⼲粉收集到管底，以避免损失。大多数蛋白可通过加入无菌蒸馏水来重新溶解。如果需要除水以外的其他稀释剂，产品 COA 中会对其进行说明。一般储备液浓度建议不要低于 100 μg/mL，分装储存避免反复冻融。

问重组蛋白有吗？

答您需要什么种属的蛋白呢，对表达系统和标签有要求吗，用于什么实验？

问重组蛋白产品该如何保存？

答1) 存储条件:重组蛋白产品收到后，如不立即使用，应当保存在 -20℃ 至 -80℃ 的条件下。冻干粉可稳定保存 12 个月以上，液体类产品在 -80℃ 条件下可保存 6-12 个月。已复溶的重组蛋白样品需尽快用完，如果不能在一周内用完，需在 -20℃ 至 -80℃ 保存，可保存 3 个月以上\n2) 避免反复融冻:重组蛋白产品应注意避免反复冻融，建议将重组蛋白产品分装成多份。分装冻存时，建议选择含有一定浓度载体蛋白的溶液或培养基复溶产品。产品浓度不低于 100 ug/mL，且每管的体积不小于 20 uL。

问蛋白能保证活性吗？

答我们有现货的产品，有活性数据的话会展示在官网生物活性那栏，没有数据的也不代表就一定没有活性，是我们还没有建立活性检测方法，没有进行测试，但是我们的蛋白都是优先按照有活性的方法去表达纯化的。

问重组蛋白产品是如何运输的？

答大部分重组蛋白产品为冻⼲粉，对温度不敏感，在室温下稳定，一般室温或蓝冰运输。对于液体类蛋白，一般采用⼲冰运输。如果您收到产品时发现冰盒已经融化，无需担心，蛋白可以正常使用，短时间内不会影响产品质量。如果特定蛋白在室温下不稳定，我们会提供相应的低温运输方式，以确保产品质量不受影响。

产品信息

生物活性	Measured by its binding ability in a functional ELISA. Immobilized mouse ERBB4-His at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1 , The EC50 of biotinylated human NRG1 is 0.55-1.29 μg/ml.
产品描述	ErbB4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 71.5 kDa and the accession number is Q99P91.
种属	Mouse
表达系统	HEK293 Cells
标签	C-His
蛋白编号	Q99P91
别名	Her4,erb-b2 receptor tyrosine kinase 4,c-erbB-4
蛋白构建	A DNA sequence encoding the mouse ERBB4 (Q99P91) (Met1-Leu652) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Gln 26
蛋白纯度	> 95 % as determined by SDS-PAGE
分子量	71.5 kDa (predicted); 113 kDa (reducing condition, due to glycosylation)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	ERBB4 is a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. ERBB4 is expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. And lower levels in thymus, lung, salivary gland, and pancreas. It specifically binds to and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. ERBB4 also can be activated by other factors and induces a variety of cellular responses including mitogenesis and differentiation. ERBB4 regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. It is required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. ERBB4 also play a role on the normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. It is required for mammary gland differentiation, induction of milk proteins and lactation.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

ErbB4 Protein, Mouse, Recombinant (His)

ErbB4 Protein, Mouse, Recombinant (His)

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