CNDP2 重组蛋白, Mouse (His)

CNDP2 Protein, Mouse, Recombinant (His)

产品编号 TMPY-01739

CNDP2 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 54.22 kDa and the accession number is Q9D1A2.

规格	价格	库存	数量
100 μg	¥ 4,460	5日内发货

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TargetMol 的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。

常见问题解答

问如何将冻干的重组蛋白配置为储备液？

答在打开盖⼦之前，先使用 10000-12000 rpm 的转速进行离心，20-30 秒，将附着在管盖或管壁上的冻⼲粉收集到管底，以避免损失。大多数蛋白可通过加入无菌蒸馏水来重新溶解。如果需要除水以外的其他稀释剂，产品 COA 中会对其进行说明。一般储备液浓度建议不要低于 100 μg/mL，分装储存避免反复冻融。

问重组蛋白产品是如何运输的？

答大部分重组蛋白产品为冻⼲粉，对温度不敏感，在室温下稳定，一般室温或蓝冰运输。对于液体类蛋白，一般采用⼲冰运输。如果您收到产品时发现冰盒已经融化，无需担心，蛋白可以正常使用，短时间内不会影响产品质量。如果特定蛋白在室温下不稳定，我们会提供相应的低温运输方式，以确保产品质量不受影响。

问重组蛋白有吗？

答您需要什么种属的蛋白呢，对表达系统和标签有要求吗，用于什么实验？

问蛋白能保证活性吗？

答我们有现货的产品，有活性数据的话会展示在官网生物活性那栏，没有数据的也不代表就一定没有活性，是我们还没有建立活性检测方法，没有进行测试，但是我们的蛋白都是优先按照有活性的方法去表达纯化的。

问重组蛋白产品该如何保存？

答1) 存储条件:重组蛋白产品收到后，如不立即使用，应当保存在 -20℃ 至 -80℃ 的条件下。冻干粉可稳定保存 12 个月以上，液体类产品在 -80℃ 条件下可保存 6-12 个月。已复溶的重组蛋白样品需尽快用完，如果不能在一周内用完，需在 -20℃ 至 -80℃ 保存，可保存 3 个月以上\n2) 避免反复融冻:重组蛋白产品应注意避免反复冻融，建议将重组蛋白产品分装成多份。分装冻存时，建议选择含有一定浓度载体蛋白的溶液或培养基复溶产品。产品浓度不低于 100 ug/mL，且每管的体积不小于 20 uL。

产品信息

生物活性	Measured by its ability to cleave carnosine (β-Ala-His) in a two step assay (mouse CNDP2 concentration 10 μg/ml). The specific activity is >40 pmoles/min/μg.
产品描述	CNDP2 Protein, Mouse, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 54.22 kDa and the accession number is Q9D1A2.
种属	Mouse
表达系统	Baculovirus Insect Cells
标签	C-His
蛋白编号	Q9D1A2
别名	Pep-1,Pep1,Dip-2,CNDP dipeptidase 2 (metallopeptidase M20 family),Cn2,C76600,0610010E05Rik
蛋白构建	A DNA sequence encoding the mouse CNDP2 (NP_075638.2) (Met 1-Asn 475) was expressed, with a C-terminal polyhistidine tag. Predicted N terminal: Met 1
蛋白纯度	> 90 % as determined by SDS-PAGE
分子量	54.22 kDa (predicted); 50 kDa (reducing conditions)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 10% glycerol, pH 8.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Cytosolic non-specific dipeptidase, also known as CNDP dipeptidase 2, Glutamate carboxypeptidase-like protein 1, Peptidase A, CNDP2 and CN2, is a cytoplasm protein which belongs to thepeptidase M2A family. CNDP2 / CPGL is a cytosolic enzyme that can hydrolyze carnosine to yield l-histidine and beta-alanine. CNDP2 / CPGL hydrolyzes a variety of dipeptides including L-carnosine but has a strong preference for Cys-Gly. Itmay be play a role as tumor suppressor in hepatocellular carcinoma (HCC) cells. Isoform1 of CNDP2 / CPGL is ubiquitously expressed with higher levels in kidney and liver (at protein level). Isoform2 of CNDP2 / CPGL is expressed in fetal tissues, it is only expressed in adult liver and placental tissues. CNDP2 / CPGL is highly expressed in the histaminergic neurons in the tuberomammillary nucleus, implying that it may supply histidine to histaminergic neurons for histamine synthesis.

CNDP2 Protein, Mouse, Recombinant (His)

CNDP2 Protein, Mouse, Recombinant (His)

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