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CD25/IL2R alpha Protein, Rat, Recombinant (His & Avi), Biotinylated

产品编号 TMPY-07247

CD25/IL2R alpha Protein, Rat, Recombinant (His & Avi), Biotinylated is expressed in Baculovirus-Insect Cells. The accession number is P26897.

CD25/IL2R alpha Protein, Rat, Recombinant (His & Avi), Biotinylated

CD25/IL2R alpha Protein, Rat, Recombinant (His & Avi), Biotinylated

产品编号 TMPY-07247
CD25/IL2R alpha Protein, Rat, Recombinant (His & Avi), Biotinylated is expressed in Baculovirus-Insect Cells. The accession number is P26897.
规格价格库存数量
20 μg
¥ 3,820
期货
100 μg
¥ 8,830
期货
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CD25/IL2R alpha Protein, Rat, Recombinant (His & Avi), Biotinylated产品问题解答
请问有 TRPA1 抑制剂吗?
HC-030031 (产品编号 T6530 CAS 349085-38-7)和 A-967079 (产品编号 T7205 CAS 1170613-55-4)。
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产品信息

生物活性
Immobilized Recombinant Rat CD25 / IL-2R alpha Protein (ECD, His & AVI Tag), Biotinylated at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human IL2 Protein, Low Endotoxin, the EC50 is 5-15 ng/mL.
产品描述
CD25/IL2R alpha Protein, Rat, Recombinant (His & Avi), Biotinylated is expressed in Baculovirus-Insect Cells. The accession number is P26897.
种属
Rat
表达系统
Baculovirus Insect Cells
标签C-His-Avi
蛋白编号P26897
别名
IL2RAC,Il2ra
蛋白构建
A DNA sequence encoding the Rat IL2RA (NP_037295.1) (Met1-Gln235) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
蛋白纯度
≥ 95% as determined by SDS-PAGE.
分子量27.72 kDa (predicted); 41.2 kDa (reducing contition, due to glycosylation)
内毒素< 1.0 EU per μg of the protein as determined by the LAL method
缓冲液Lyophilized from sterile 20 mM Tris, 300 mM NaCl, 10% Glycerol, pH 7.5. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
复溶方法
Please refer to the lot-specific COA.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
CD25 (alpha-chain of the IL-2 receptor, or IL2RA), is a type I transmembrane glycoprotein with a signal peptide, an extracellular region, a transmembrane region, and a cytoplasmic domain. IL2RA is expressed on activated T cells and regulatory T cells and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high-affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covalently with the IL2 receptor beta and gamma chain, and subsequently initiates the intracellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling. The IL2RA (CD25) gene is a substantial component of the high-affinity receptor molecule highly expressed by activated T lymphocytes. Recently, a piece of strong evidence was obtained for the involvement of IL-2RA in conferring susceptibility to type 1 diabetes (T1D). Cancer growth and development are associated with the stimulation of the innate immune system, including enhanced interleukin 2 receptor (IL-2R) expression in immune cells and its shedding into the circulation in a soluble form of SIL-2Ralpha. In most hematological malignancies, including different types of leukemias and lymphomas, SIL-2Ralpha is released directly from the surface of neoplastic cells thus reflecting the tumor bulk, turnover, and activity. Several studies have proved that not only lymphoid cancer cells but also some non-lymphoid cancer cells, express IL-2R on their surface. They include malignant melanoma and carcinomas of the kidney, head and neck, esophagus, and lung. Thus, sIL-2Ralpha is elevated in most proliferative disturbances of the hematopoietic system and many solid tumors.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

SCI 文献

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