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Carbonic Anhydrase 4 Protein, Human, Recombinant (His), Biotinylated

产品编号 TMPY-07021Carbonic Anhydrase 4 Protein, Human, Recombinant (His), Biotinylated

Carbonic Anhydrase 4 Protein, Human, Recombinant (His), Biotinylated is expressed in CHO Stable Cells. The accession number is P22748-1.

Carbonic Anhydrase 4 Protein, Human, Recombinant (His), Biotinylated

Carbonic Anhydrase 4 Protein, Human, Recombinant (His), Biotinylated

产品编号 TMPY-07021Carbonic Anhydrase 4 Protein, Human, Recombinant (His), Biotinylated
Carbonic Anhydrase 4 Protein, Human, Recombinant (His), Biotinylated is expressed in CHO Stable Cells. The accession number is P22748-1.
规格价格库存数量
20 μg
¥ 3,820
期货
100 μg
¥ 8,830
期货
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常见问题解答
抑制剂能否用于动物实验?
可以的,我们的抑制剂都可以用于动物/体内实验。但有些化合物还没有用于动物实验的文献,这种情况下,建议您先做预实验,确保研究的可行性。
抑制剂是否可以高温灭菌?
大部分产品均通过化学方法合成,反应条件一般在 50-80℃ 之间,因此不建议使用高温灭菌法进行灭菌,建议用 0.22 μm 滤膜过滤灭菌。
期货产品没有纯度啊?这个纯度在哪里看
期货产品的话,是没有纯度信息,也没有质检信息的。有了现货之后我们会进行质检,测出试剂的纯度和结构等相关信息,并且展示在官网的活性描述上方。
收到粉状试剂粘壁严重,如何处理?
建议您用离心机 3000 rpm下离心一会儿,将粉末收集到管底。
抑制剂是否需要灭菌?
如果您使用 DMSO 配制:不建议灭菌,DMSO 本身具有极强的杀菌力,配制好的溶液就是无菌溶液。如果您确实担心的,可以放置 4℃ 冰箱过夜放置即可;依然有顾虑的话,需用有机系专用滤膜过滤。 如果您使用水配制:可以用 0.22 μm 滤膜过滤灭菌。
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产品信息

生物活性
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
Carbonic Anhydrase 4 Protein, Human, Recombinant (His), Biotinylated is expressed in CHO Stable Cells. The accession number is P22748-1.
种属
Human
表达系统
CHO Cells
标签C-His
蛋白编号P22748-1
别名
RP17,Car4,CAIV
蛋白构建
A DNA sequence encoding the human CA4 (NP_000708.1) (Met1-Lys283) without the pro peptide was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.
蛋白纯度
≥ 90% as determined by SDS-PAGE.
分子量31.69 kDa (predicted); 33 kDa (reducing contition)
内毒素< 1.0 EU per μg protein as determined by the LAL method.
缓冲液Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
复溶方法
Please refer to the lot-specific COA.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3-dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.

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