Carbonic Anhydrase 4 Protein, Human, Recombinant (His), Biotinylated is expressed in CHO Stable Cells. The accession number is P22748-1.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 20 μg | ¥ 3,820 | 期货 | |
| 100 μg | ¥ 8,830 | 期货 |
| 生物活性 | Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
| 产品描述 | Carbonic Anhydrase 4 Protein, Human, Recombinant (His), Biotinylated is expressed in CHO Stable Cells. The accession number is P22748-1. |
| 种属 | Human |
| 表达系统 | CHO Cells |
| 标签 | C-His |
| 蛋白编号 | P22748-1 |
| 别名 | RP17,Car4,CAIV |
| 蛋白构建 | A DNA sequence encoding the human CA4 (NP_000708.1) (Met1-Lys283) without the pro peptide was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro. |
| 蛋白纯度 | ≥ 90% as determined by SDS-PAGE. |
| 分子量 | 31.69 kDa (predicted); 33 kDa (reducing contition) |
| 内毒素 | < 1.0 EU per μg protein as determined by the LAL method. |
| 缓冲液 | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| 复溶方法 | Please refer to the lot-specific COA. |
| 存储 | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. |
| 研究背景 | The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3-dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII. |
嗨！有任何问题？点我咨询
