BID Protein, Mouse, Recombinant (His)

Bid, BH3-interacting domain death agonist, was initially cloned based in its ability to interact with both Bcl-2 and Bax. Bid contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. Bid is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Bid is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals.

规格	价格/CNY		数量
100 μg	￥ 3,400	5日内发货
500 μg	￥ 13,600	5日内发货
1 mg	￥ 22,700	5日内发货

规格

价格/CNY

货期

数量

100 μg

￥ 3,400

5日内发货

500 μg

￥ 13,600

5日内发货

1 mg

￥ 22,700

5日内发货

生物活性

Mouse BID, His Tag immobilized on CM5 Chip can bind Human BCL2L1/Bcl-XL Protein, His Tag with an affinity constant of 33.06 nM as determined in SPR assay.

产品描述

种属	Mouse
表达系统	E. coli
标签	C-His
蛋白编号	EDK99650.1
别名	BID, p22 BID
蛋白构建	Recombinant Mouse BID Protein is expressed from E.coli with His tag at the C-Terminus. It contains Ala1-Ser195.[Accession \|EDK99650.1]
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 90% as determined by HPLC
分子量	The protein has a predicted MW of 22.72 kDa same as Tris-Bis PAGE result.

内毒素	Less than 1EU per μg by the LAL method.
缓冲液	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
存储	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
运输方式	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
研究背景	Bid, BH3-interacting domain death agonist, was initially cloned based in its ability to interact with both Bcl-2 and Bax. Bid contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. Bid is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Bid is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals.