BID Protein, Mouse, Recombinant (His)

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SPR兼容缓冲液

产品编号 TMPK-00636

Bid, BH3-interacting domain death agonist, was initially cloned based in its ability to interact with both Bcl-2 and Bax. Bid contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. Bid is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Bid is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals.

BID Protein, Mouse, Recombinant (His)

一键复制产品信息

SPR兼容缓冲液

产品编号 TMPK-00636

规格	价格	库存
5 μg	¥ 388	6-8日内发货
10 μg	¥ 625	6-8日内发货
20 μg	¥ 987	5日内发货
50 μg	¥ 1,980	5日内发货
100 μg	¥ 3,400	5日内发货
200 μg	¥ 6,160	5日内发货
500 μg	¥ 13,600	5日内发货

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产品信息

生物活性	Mouse BID, His Tag immobilized on CM5 Chip can bind Human BCL2L1/Bcl-XL Protein, His Tag with an affinity constant of 33.06 nM as determined in SPR assay.
产品描述	Bid, BH3-interacting domain death agonist, was initially cloned based in its ability to interact with both Bcl-2 and Bax. Bid contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. Bid is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Bid is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals.
种属	Mouse
表达系统	E. coli
标签	C-His
蛋白编号	EDK99650.1
别名	p22 BID,BID
蛋白构建	Ala1-Ser195
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 90% as determined by HPLC
分子量	22.72 kDa (predicted) same as Tris-Bis PAGE result.
内毒素	< 1 EU/μg by the LAL method.
蛋白性状	Lyophilized powder
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Bid, BH3-interacting domain death agonist, was initially cloned based in its ability to interact with both Bcl-2 and Bax. Bid contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. Bid is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Bid is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals.