B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. B7-H5/Gi24/VISTA Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 19 kDa and the accession number is Q9H7M9.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 2,360 | 5日内发货 | ||
500 μg | ¥ 9,490 | 5日内发货 | ||
1 mg | ¥ 15,800 | 5日内发货 |
生物活性 | Immobilized Human B7-H5, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA. |
产品描述 | B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. B7-H5/Gi24/VISTA Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 19 kDa and the accession number is Q9H7M9. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | Q9H7M9 |
别名 | VSIR, B7H5, 4632428N05Rik, SISP1, PP2135, Dies1, PD-1H, VISTA, C10orf54, Gi24, PD1H, B7-H5 |
蛋白构建 | Phe33-Ala194 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 19 kDa (predicted). Due to glycosylation, the protein migrates to 32-70 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | B7-H5, also known as VISTA , B7H5, Dies1, SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. |
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B7-H5/Gi24/VISTA Protein, Human, Recombinant (His) VSIR B7H5 4632428N05Rik SISP 1 SISP1 PP2135 Dies1 PD-1H VISTA C10orf54 Gi24 PD1H PP-2135 B7-H5 SISP-1 PP 2135 recombinant recombinant-proteins proteins protein