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B7-2 Protein, Rhesus macaque, Recombinant (His)

产品编号 TMPJ-00641

B7-2 Protein, Rhesus macaque, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 40-90 KDa and the accession number is H9ZFI8.

B7-2 Protein, Rhesus macaque, Recombinant (His)

B7-2 Protein, Rhesus macaque, Recombinant (His)

产品编号 TMPJ-00641
B7-2 Protein, Rhesus macaque, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 40-90 KDa and the accession number is H9ZFI8.
规格价格库存数量
10 μg
¥ 380
5日内发货
50 μg
¥ 900
5日内发货
500 μg
¥ 4,540
5日内发货
1 mg
¥ 6,520
5日内发货
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TargetMol 的所有产品仅用作科学研究或药证申报,不能被用于人体,我们不向个人提供产品和服务。请您遵守承诺用途,不得违反法律法规规定用于任何其他用途。
实验操作小课堂
常见问题解答
加药后检测结果没有作用,是不是产品质量有问题?
产品质量没有问题,都是经过两次严格质检的,HNMR确定产品结构,HPLC确定化合物纯度。同一个抑制剂由于实验材料(细胞、动物)的不同,IC50 和实验效果也是不一样的,不能照搬文献报道的方法,应该根据自己的实验设立浓度梯度,得到一个最适合的浓度。
PEG300 可以用 PEG400 替代吗?
PEG300 具有适度的粘稠度,有利于药物的溶解和给药过程,同时也能够提供良好的耐受性,不会对动物造成不良影响。PEG400 可以替代 PEG300。 PEG600 不是很建议,因为熔点接近常温。
抑制剂加 DMSO 配成母液之后,能否放在常温的环境保存1周?
不建议这么做,母液配置好分装保存,建议存于 -20℃ 或 -80℃,尽量避免反复冻融。如果您订购的规格很大,可以考虑分装粉末保存,固体粉末是比较稳定的,-20℃ 可以保存三年。
超声久了会不会对化合物的结构有影响?
建议以较低频率对化合物进行超声,不会对化合物的结构造成影响。
化合物如何用于动物实验?如何确立剂量、给药方式等?
同一个化合物对不同动物模型、不同疾病模型的用法不一样,建议根据实验目的,查询文献选择用法。 常见的给药方式包括腹腔注射、灌胃等。由于首过效应,一般可以灌胃的也可以腹腔注射,可以腹腔注射的不一定适合灌胃。不同种属的给药剂量可以根据官网的表格换算。但仅供参考,实际应用中可能未必合适或者准确,尽量以具体某种动物给药剂量的参考文献为主。 我们的化合物都可以进行细胞动物实验,但有些化合物还没有用于动物实验的文献,这种情况下,您可以尝试进行动物实验,但我们无法提供使用方式,也无法保证药效。
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产品信息

生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
B7-2 Protein, Rhesus macaque, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 40-90 KDa and the accession number is H9ZFI8.
种属
Rhesus
表达系统
HEK293 Cells
标签C-6xHis
蛋白编号H9ZFI8
别名
T-lymphocyte activation antigen CD86 isoform 1,CD86,Activation B7-2 antigen
氨基酸序列
Tyr31-Pro247
蛋白构建
Tyr31-Pro247
蛋白纯度
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
分子量40-90 KDa (reducing condition)
内毒素< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
复溶方法
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
T-lymphocyte activation antigen CD86 (B7-2) is a glycosylated protein in the B7 family. B7 family members are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance. It is highly expressed on activated antigen presenting cells. CD86 involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. It may play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. It is expressed by activated B-lymphocytes and monocytes and promoted by MARCH8 and results in endocytosis and lysosomal degradation.

参考文献

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